人类非淋巴组织 DC 的制备和流式细胞术分析指南。

IF 4.5 3区 医学 Q2 IMMUNOLOGY
Diana Dudziak, Lukas Heger, William W Agace, Joyce Bakker, Tanja D de Gruijl, Regine J Dress, Charles-Antoine Dutertre, Thomas M Fenton, Marieke F Fransen, Florent Ginhoux, Oded Heyman, Yael Horev, Florian Hornsteiner, Vinitha Kandiah, Paz Kles, Ruth Lubin, Gabriel Mizraji, Anastasia Prokopi, Or Saar, Sieghart Sopper, Patrizia Stoitzner, Helen Strandt, Martina M Sykora, Elisa C Toffoli, Christoph H Tripp, Kim van Pul, Rieneke van de Ven, Asaf Wilensky, Simon Yona, Claudia Zelle-Rieser
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摘要

本文章由计算机程序翻译,如有差异,请以英文原文为准。
Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC.

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.

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来源期刊
CiteScore
8.30
自引率
3.70%
发文量
224
审稿时长
2 months
期刊介绍: The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.
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