Honoka Nojo, Nobuo Murayama, Sanae A Ishijima, Rui Kano
{"title":"In vitro susceptibility testing of essential oils and their main components against antifungal-resistant Malassezia pachydermatis.","authors":"Honoka Nojo, Nobuo Murayama, Sanae A Ishijima, Rui Kano","doi":"10.1292/jvms.24-0342","DOIUrl":null,"url":null,"abstract":"<p><p>Canine Malassezia dermatitis (CMD) and otitis externa are generally treated by antifungal drugs. However, azole-resistant strains have been isolated from canine skin and ear canals worldwide. Phytochemicals isolated from essential oils are effective alternatives for inhibiting Malassezia pachydermatis. To evaluate the usefulness of phytochemicals against azole-resistant isolates, we performed in vitro susceptibility testing using the phytochemicals carvacrol, citral, and thymol. Eight antifungal-resistant isolates were obtained from 7 cases of dermatitis and 1 case of otitis externa during 2022 and 2023 from dogs in Tokyo and Kanagawa, Japan. Fungal susceptibility to carvacrol, citral, and thymol was assessed using the modified broth microdilution method. The minimum inhibitory concentrations (MICs) of the phytochemicals in all isolates were as follows: 0.03 to 0.125% for carvacrol; 0.03 to 0.125% for thymol; and 0.03% to 0.125% for citral. Based on these results, carvacrol, citral, and thymol appear to be effective against azole-resistant strains. The phytochemicals appear to be effective for treating antifungal-resistant cases of CMD and otitis externa.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.24-0342","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
In vitro susceptibility testing of essential oils and their main components against antifungal-resistant Malassezia pachydermatis.
Canine Malassezia dermatitis (CMD) and otitis externa are generally treated by antifungal drugs. However, azole-resistant strains have been isolated from canine skin and ear canals worldwide. Phytochemicals isolated from essential oils are effective alternatives for inhibiting Malassezia pachydermatis. To evaluate the usefulness of phytochemicals against azole-resistant isolates, we performed in vitro susceptibility testing using the phytochemicals carvacrol, citral, and thymol. Eight antifungal-resistant isolates were obtained from 7 cases of dermatitis and 1 case of otitis externa during 2022 and 2023 from dogs in Tokyo and Kanagawa, Japan. Fungal susceptibility to carvacrol, citral, and thymol was assessed using the modified broth microdilution method. The minimum inhibitory concentrations (MICs) of the phytochemicals in all isolates were as follows: 0.03 to 0.125% for carvacrol; 0.03 to 0.125% for thymol; and 0.03% to 0.125% for citral. Based on these results, carvacrol, citral, and thymol appear to be effective against azole-resistant strains. The phytochemicals appear to be effective for treating antifungal-resistant cases of CMD and otitis externa.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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