Wanhao Li, Jiali Xing, Jinzhong Xi, Shaofeng Yuan, Zhilong Yu, Hang Yu, Yuliang Cheng, Yahui Guo, Yunfei Xie and Weirong Yao
{"title":"使用具有自动复位功能的三向DNA结放大器进行葡萄球菌肠毒素B的无酶免疫吸附测定","authors":"Wanhao Li, Jiali Xing, Jinzhong Xi, Shaofeng Yuan, Zhilong Yu, Hang Yu, Yuliang Cheng, Yahui Guo, Yunfei Xie and Weirong Yao","doi":"10.1039/D4AN01370K","DOIUrl":null,"url":null,"abstract":"<p >A highly sensitive immunoadsorption assay without traditional horseradish peroxidase for signal amplification was developed, utilizing a three-way DNA junction amplifier instead. The formation of a three-way DNA junction and release of trigger DNA with recycling was accomplished by toehold-mediated strand displacement. The fluorescent dye <em>N</em>-methyl mesoporphyrin IX, with highly structure-specific binding affinity towards G-quadruplexes, was employed for label-free and simple signal output. High sensitivity was achieved for concentrations as low as 7.21 pg mL<small><sup>−1</sup></small> by targeting Staphylococcal enterotoxin B for detection, and its applicability was confirmed by a recovery study of precooked dishes.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 4","pages":" 605-611"},"PeriodicalIF":3.6000,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An enzyme-free immunosorbent assay of staphylococcal enterotoxin B using a three-way DNA junction amplifier with an automatic reset function†\",\"authors\":\"Wanhao Li, Jiali Xing, Jinzhong Xi, Shaofeng Yuan, Zhilong Yu, Hang Yu, Yuliang Cheng, Yahui Guo, Yunfei Xie and Weirong Yao\",\"doi\":\"10.1039/D4AN01370K\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >A highly sensitive immunoadsorption assay without traditional horseradish peroxidase for signal amplification was developed, utilizing a three-way DNA junction amplifier instead. The formation of a three-way DNA junction and release of trigger DNA with recycling was accomplished by toehold-mediated strand displacement. The fluorescent dye <em>N</em>-methyl mesoporphyrin IX, with highly structure-specific binding affinity towards G-quadruplexes, was employed for label-free and simple signal output. High sensitivity was achieved for concentrations as low as 7.21 pg mL<small><sup>−1</sup></small> by targeting Staphylococcal enterotoxin B for detection, and its applicability was confirmed by a recovery study of precooked dishes.</p>\",\"PeriodicalId\":63,\"journal\":{\"name\":\"Analyst\",\"volume\":\" 4\",\"pages\":\" 605-611\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-12-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analyst\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/an/d4an01370k\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analyst","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/an/d4an01370k","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
An enzyme-free immunosorbent assay of staphylococcal enterotoxin B using a three-way DNA junction amplifier with an automatic reset function†
A highly sensitive immunoadsorption assay without traditional horseradish peroxidase for signal amplification was developed, utilizing a three-way DNA junction amplifier instead. The formation of a three-way DNA junction and release of trigger DNA with recycling was accomplished by toehold-mediated strand displacement. The fluorescent dye N-methyl mesoporphyrin IX, with highly structure-specific binding affinity towards G-quadruplexes, was employed for label-free and simple signal output. High sensitivity was achieved for concentrations as low as 7.21 pg mL−1 by targeting Staphylococcal enterotoxin B for detection, and its applicability was confirmed by a recovery study of precooked dishes.
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