Low humidity enhances thermotolerance in Caenorhabditis elegans.

Humidity is an important environmental factor that causes physiological changes in organisms. In humans, high humidity disrupts thermoregulation by limiting heat dissipation, leading to heat stress. While Caenorhabditis elegans lacks comparable thermoregulatory systems, humidity may still impact its heat tolerance by affecting cellular stress responses. We tested this by subjecting C. elegans to heat shock under different humidity conditions and found that lower humidity during heat shock improved survival compared to higher humidity. These findings demonstrate that humidity is an important variable affecting thermotolerance in C. elegans and should be standardized in heat-stress experiments.