中药如何提高抗生素治疗mrsa感染肺炎的疗效：热炎宁合剂与利奈唑胺联合应用的实验研究。

IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of ethnopharmacology Pub Date : 2025-01-31 DOI:10.1016/j.jep.2024.119221

Min-Hang Dou, Jia-Yi Huang, Peng-Yue Li, Wan-Ling Chen, Xin-Ran Wang, Tian-Zi Yang, Xiao-Yu Fan, Xin-Yu Zhang, Yang Lu, Jie Bai, Shou-Ying Du

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引用次数: 0

摘要

民族药理意义：热炎宁合剂（RYN）是中国广泛用于治疗呼吸道炎症性疾病的中成药，在治疗细菌性肺炎方面具有潜力。研究目的：本研究旨在证明RYN联合利奈唑胺治疗mrsa感染的肺炎的治疗潜力，并探讨其作用机制和有效成分。材料与方法：在mrsa引起的肺炎大鼠模型中，研究RYN单用及联用利奈唑胺的药效学。采用转录组学、ELISA、western blot、qRT-PCR等方法，探索验证RYN抗炎作用的药理机制。采用UPLC-MS和分子对接方法，探索RYN治疗mrsa感染肺炎的抗炎成分。结果：在体内，RYN可减轻肺炎患者的肺损伤和炎症反应。特别是，RYN和利奈唑胺联合使用比单独使用任何一种治疗都能提高治疗效果。进一步研究表明，联合用药的协同治疗效果可能与RYN抑制炎症反应、增强利奈唑胺对肺组织MRSA的抑制和清除作用有关。RYN通过抑制TLR2/NF-κB/NLRP3通路在mrsa感染的肺炎中发挥抗炎作用，有7种活性成分可能起主导作用。结论：这些结果提示RYN可能作为抗生素治疗mrsa相关性肺炎的辅助药物。探索其有效成分和作用机制，有利于RYN的临床应用和质量的提高。更重要的是，本研究表明，中药与抗生素联合治疗的协同治疗效果可能是一种有价值的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

How can traditional Chinese medicine enhance the efficacy of antibiotics in the treatment of MRSA-infected pneumonia: An experimental study on the combination of Reyanning mixture (RYN) and linezolid

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How can traditional Chinese medicine enhance the efficacy of antibiotics in the treatment of MRSA-infected pneumonia: An experimental study on the combination of Reyanning mixture (RYN) and linezolid

Ethnopharmacological relevance

The Reyanning Mixture (RYN) is a Chinese patent medicine widely used in the treatment of respiratory inflammatory diseases in China and has potential in the treatment of bacteria-infected pneumonia.

Aim of the study

The present study aimed to demonstrate the therapeutic potential of RYN in combination with linezolid for the treatment of MRSA-infected pneumonia and to explore the mechanisms of action and active components.

Materials and methods

The pharmacodynamics of RYN alone and in combination with linezolid was investigated in a rat model of MRSA-induced pneumonia. Transcriptomics, ELISA, Western blot and qRT-PCR were used to explore and verify the pharmacological mechanism of the anti-inflammatory effect of RYN. UPLC-MS and molecular docking were used to explore the anti-inflammatory components of RYN for the treatment of MRSA-infected pneumonia.

Results

In vivo, RYN reduced lung injury and inflammation in rats with pneumonia. In particular, the combination of RYN and linezolid enhanced the therapeutic effect compared to that of either treatment alone. Further research suggests that the synergistic therapeutic effect of the combination may be related to the inhibition of the inflammatory response by RYN and the enhancement of linezolid inhibition and clearance of MRSA in lung tissues by RYN. RYN plays an anti-inflammatory role in MRSA-infected pneumonia by inhibiting the TLR2/NF-κB/NLRP3 pathway, with 7 active components that may play a dominant role.

Conclusions

These results indicate that RYN may serve as an adjuvant drug to antibiotics for the treatment of MRSA-associated pneumonia. Exploration of its mechanisms and active components is conducive to the clinical application and quality improvement of RYN. More importantly, this study showed that the synergistic therapeutic effect of the combination of traditional Chinese medicine and antibiotics may be a valuable therapeutic strategy.

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.