{"title":"印度辅助生殖技术妊娠中胎盘血管生成因子的表达和甲基化。","authors":"Deepali Sundrani, Aishwarya Kapare, Himanshi Yadav, Karuna Randhir, Sanjay Gupte, Sadhana Joshi","doi":"10.1080/17501911.2024.2438593","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>This study aims to examine the gene expression and DNA methylation patterns of angiogenic factors in the placentae of Indian women who underwent assisted reproductive technology (ART) procedures and their association with maternal one-carbon metabolites and birth outcome.</p><p><strong>Methods: </strong>Placental gene expression and DNA methylation of angiogenic factors (<i>VEGF</i>, <i>PlGF</i>, <i>FLT-1</i>, <i>KDR</i>) in Indian women who underwent ART procedures (<i>n</i> = 64) and women who conceived naturally (Non-ART) (<i>n</i> = 93) was investigated using RT-qPCR and Epitect Methyl-II PCR assay kits. Maternal plasma one-carbon metabolites were assessed by CMIA technology.</p><p><strong>Result: </strong>Gene expression of <i>FLT-1</i> and <i>KDR</i> was higher (<i>p</i> < 0.05) in the ART placentae. Placental global DNA methylation levels were higher (<i>p</i> < 0.01) and DNA methylation levels of <i>VEGF</i> promoter were lower (<i>p</i> < 0.05) in ART compared to non-ART women. Maternal plasma folate and vitamin B<sub>12</sub> levels were higher (<i>p</i> < 0.01) in the ART group. Gene expression of <i>PlGF</i> was negatively associated with maternal plasma folate (<i>p</i> < 0.05) whereas KDR was positively associated with maternal plasma homocysteine (<i>p</i> < 0.05). Gene expression of <i>KDR</i> was positively associated with chest circumference of the baby (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Hypomethylation of <i>VEGF</i> and increased expression of <i>FLT-1</i> and <i>KDR</i> was observed in the placentae of women who underwent ART procedure.</p>","PeriodicalId":11959,"journal":{"name":"Epigenomics","volume":" ","pages":"21-31"},"PeriodicalIF":3.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Placental expression and methylation of angiogenic factors in assisted reproductive technology pregnancies from India.\",\"authors\":\"Deepali Sundrani, Aishwarya Kapare, Himanshi Yadav, Karuna Randhir, Sanjay Gupte, Sadhana Joshi\",\"doi\":\"10.1080/17501911.2024.2438593\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>This study aims to examine the gene expression and DNA methylation patterns of angiogenic factors in the placentae of Indian women who underwent assisted reproductive technology (ART) procedures and their association with maternal one-carbon metabolites and birth outcome.</p><p><strong>Methods: </strong>Placental gene expression and DNA methylation of angiogenic factors (<i>VEGF</i>, <i>PlGF</i>, <i>FLT-1</i>, <i>KDR</i>) in Indian women who underwent ART procedures (<i>n</i> = 64) and women who conceived naturally (Non-ART) (<i>n</i> = 93) was investigated using RT-qPCR and Epitect Methyl-II PCR assay kits. Maternal plasma one-carbon metabolites were assessed by CMIA technology.</p><p><strong>Result: </strong>Gene expression of <i>FLT-1</i> and <i>KDR</i> was higher (<i>p</i> < 0.05) in the ART placentae. Placental global DNA methylation levels were higher (<i>p</i> < 0.01) and DNA methylation levels of <i>VEGF</i> promoter were lower (<i>p</i> < 0.05) in ART compared to non-ART women. Maternal plasma folate and vitamin B<sub>12</sub> levels were higher (<i>p</i> < 0.01) in the ART group. Gene expression of <i>PlGF</i> was negatively associated with maternal plasma folate (<i>p</i> < 0.05) whereas KDR was positively associated with maternal plasma homocysteine (<i>p</i> < 0.05). Gene expression of <i>KDR</i> was positively associated with chest circumference of the baby (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Hypomethylation of <i>VEGF</i> and increased expression of <i>FLT-1</i> and <i>KDR</i> was observed in the placentae of women who underwent ART procedure.</p>\",\"PeriodicalId\":11959,\"journal\":{\"name\":\"Epigenomics\",\"volume\":\" \",\"pages\":\"21-31\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Epigenomics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/17501911.2024.2438593\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/17501911.2024.2438593","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/10 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
摘要
目的：本研究旨在研究接受辅助生殖技术（ART）手术的印度妇女胎盘血管生成因子的基因表达和DNA甲基化模式及其与母体一碳代谢物和分娩结局的关系。方法：采用RT-qPCR和Epitect Methyl-II PCR检测试剂盒，对64例接受ART治疗的印度妇女（n = 64）和93例自然受孕的印度妇女（n = 93）胎盘血管生成因子（VEGF、PlGF、FLT-1、KDR）的基因表达和DNA甲基化进行了研究。采用CMIA技术检测母体血浆单碳代谢物。结果：FLT-1和KDR基因表达较高(p p VEGF启动子较低（p p 12水平较高）PlGF与母体血浆叶酸呈负相关(p p KDR与婴儿胸围呈正相关(p)结论：在接受ART手术的妇女胎盘中观察到VEGF低甲基化和FLT-1和KDR表达升高。
Placental expression and methylation of angiogenic factors in assisted reproductive technology pregnancies from India.
Aim: This study aims to examine the gene expression and DNA methylation patterns of angiogenic factors in the placentae of Indian women who underwent assisted reproductive technology (ART) procedures and their association with maternal one-carbon metabolites and birth outcome.
Methods: Placental gene expression and DNA methylation of angiogenic factors (VEGF, PlGF, FLT-1, KDR) in Indian women who underwent ART procedures (n = 64) and women who conceived naturally (Non-ART) (n = 93) was investigated using RT-qPCR and Epitect Methyl-II PCR assay kits. Maternal plasma one-carbon metabolites were assessed by CMIA technology.
Result: Gene expression of FLT-1 and KDR was higher (p < 0.05) in the ART placentae. Placental global DNA methylation levels were higher (p < 0.01) and DNA methylation levels of VEGF promoter were lower (p < 0.05) in ART compared to non-ART women. Maternal plasma folate and vitamin B12 levels were higher (p < 0.01) in the ART group. Gene expression of PlGF was negatively associated with maternal plasma folate (p < 0.05) whereas KDR was positively associated with maternal plasma homocysteine (p < 0.05). Gene expression of KDR was positively associated with chest circumference of the baby (p < 0.05).
Conclusion: Hypomethylation of VEGF and increased expression of FLT-1 and KDR was observed in the placentae of women who underwent ART procedure.
期刊介绍：
Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community.
Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
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