两种诱导多能干细胞系的健康对照。

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-02-01 DOI:10.1016/j.scr.2024.103621

Dan Zhang , Di Huang , Leon M. Larcher , Khine Zaw , Shang-Chih Chen , Luke Jennings , Tina M. Lamey , Jennifer A. Thompson , Terri L. McLaren , Fred K. Chen , Samuel McLenachan

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引用次数: 0

摘要

两种人类诱导多能干细胞系LEIi021-A和LEIi021-B，来源于来自澳大利亚土著视网膜色素性视网膜炎-11家族的健康对照受试者的真皮成纤维细胞。使用表达OCT4、SOX2、LIN28、L-MYC、KLF4和mp53DD的episal载体进行重编程。多能性标记在LEIi021-A和LEIi021-B系中均有表达。这两种细胞系核型正常，并能分化为具有三个初级胚层的胚状体和视网膜色素上皮细胞。

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Derivation of two induced pluripotent stem cell lines from a healthy control subject

Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.