用超分辨率显微镜分析病毒结构的复杂性

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2024-12-09 DOI:10.1007/s00705-024-06192-3

Kevin Olaya-Bravo, Daniel Martínez-Flores, Aaron Pavel Rodríguez-Hernández, Ileana Tobías-Juárez, Jorge A. Castro-Rodríguez, Alicia Sampieri, Luis Vaca

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引用次数: 0

摘要

在这篇综述中，我们讨论了不同的超分辨率显微镜（SRM）技术用于研究纳米分辨率的病毒结构和病毒组成。我们描述了用于打破光衍射极限的不同显微镜方法的基本原理，使研究病毒结构中的蛋白质组成成为可能。最后，我们首次使用单分子超分辨率显微镜证明了两种结构蛋白在单个杆状病毒中的差异空间分布。我们将讨论这些强有力的方法在病毒学、医学和生物技术方面的应用前景。

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Resolving viral structural complexity by super-resolution microscopy

In this review, we discuss different super-resolution microscopy (SRM) techniques employed to study viral structures and virus composition with nanometric resolution. We describe the basic principles of the different microscopy methods utilized to break the light diffraction limit, enabling the study of protein composition in viral structures. Finally, we demonstrate for the first time the differential spatial distribution of two structural proteins in an individual baculovirus using single-molecule super-resolution microscopy. We discuss the future of these powerful methods for virology, medicine, and biotechnology applications.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.