一种简单合成的银离子掺杂多孔金微粒SERS适体传感器，用于超灵敏和宽范围定量检测IL-6

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-01-22 DOI:10.1016/j.aca.2024.343504

Bixia Liang , Zhixi Huang , Lingyan Cheng , Jin Quan, Zongxia Mou, Nan Li, Xin Cui

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引用次数: 0

摘要

多功能细胞因子白细胞介素-6 （IL-6）在慢性和急性炎症反应中起关键作用，强调准确测定IL-6水平对早期诊断、预防和治疗炎症的重要性。本研究开发了一种多功能和创新的单粒子表面增强拉曼光谱（SERS）传感平台，用于精确和敏感地定量复杂样品中的IL-6，使用一种新型的单锅合成，银离子掺杂三维多孔金微粒子（PGMs），具有丰富的热点，具有强大的SERS增强。通过合理设计IL-6适体与PGMs互补链之间丰富的胞嘧啶- ag +-胞嘧啶碱基对，利用sers增强效应，在10-9 ~ 10-4 mg/mL范围内实现了IL-6的高灵敏度和特异性定量，检出限（LOD）为0.093 pg/mL。此外，加样血清中IL-6的准确检出率为97.2% ~ 107.6%。该适体传感器提供了一种成本效益高、灵敏度高、范围广的IL-6检测替代工具，有助于早期发现炎症，为及时干预和改善患者预后铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A simply synthesized, silver ions-doped porous gold microparticles-based SERS aptamer sensor for ultrasensitive and broad-range quantitative detection of IL-6

查看原文本刊更多论文

A simply synthesized, silver ions-doped porous gold microparticles-based SERS aptamer sensor for ultrasensitive and broad-range quantitative detection of IL-6

Background

The multifunctional cytokine interleukin-6 (IL-6) plays a pivotal role in chronic and acute inflammatory responses, underscoring the importance of accurately determining IL-6 levels for early diagnosis, prevention, and treatment of inflammation.

Results

This study developed a versatile and innovative single-particle surface-enhanced Raman spectroscopy (SERS) sensing platform for the precise and sensitive quantification of IL-6 in complex samples using a novel one-pot synthesized, silver ions-doped three-dimensional porous gold microparticles (PGMs) with abundant hot spots for robust SERS enhancement. By rationally designing rich cytosine-Ag⁺-cytosine base pairs between IL-6 aptamers and complementary chains on the PGMs, we harnessed the SERS-enhancing effect to achieve highly sensitive and specific IL-6 quantification within a wide range of 10⁻⁹ to 10⁻⁴ mg/mL and a limit of detection (LOD) of 0.093 pg/mL. Moreover, the accurate detection of IL-6 in spiked serum samples exhibited a remarkable recovery rate ranging from 97.2 % to 107.6 %.

Significance

This aptamer sensor provides an alternative tool for cost-effective, sensitive and broad-range IL-6 detection, which would contribute to the early detection of inflammation and pave the way for timely interventions and improved patient outcomes.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.