Diana Munguia-Ramos, Luis Fernando Xancal-Salvador, Verónica Esteban-Kenel, Narciso Ortiz-Conchi, Ricardo Antonio Jaimes-Aquino, Miguel Mendoza-Rojas, Axel Cervantes-Sánchez, Steven Méndez-Ramos, Hector Orlando Rivera-Villegas, Sandra Rajme-Lopez, Karla Maria Tamez-Torres, Carla Marina Roman-Montes, Areli Martínez-Gamboa, Miriam Bobadilla Del-Valle, Jose Sifuentes-Osornio, Alfredo Ponce-de-Leon, Maria Fernanda Gonzalez-Lara, Bernardo Alfonso Martinez-Guerra
{"title":"使用 NG-Test® CARBA 5 在阳性血液培养物中快速检测碳青霉烯酶：诊断测试研究。","authors":"Diana Munguia-Ramos, Luis Fernando Xancal-Salvador, Verónica Esteban-Kenel, Narciso Ortiz-Conchi, Ricardo Antonio Jaimes-Aquino, Miguel Mendoza-Rojas, Axel Cervantes-Sánchez, Steven Méndez-Ramos, Hector Orlando Rivera-Villegas, Sandra Rajme-Lopez, Karla Maria Tamez-Torres, Carla Marina Roman-Montes, Areli Martínez-Gamboa, Miriam Bobadilla Del-Valle, Jose Sifuentes-Osornio, Alfredo Ponce-de-Leon, Maria Fernanda Gonzalez-Lara, Bernardo Alfonso Martinez-Guerra","doi":"10.3390/antibiotics13111105","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Infections due to carbapenem-resistant Gram-negative bacteria are emerging as an important challenge in health-care settings and a growing concern worldwide. Lateral flow immunoassay NG-Test<sup>®</sup> CARBA 5 can detect the five most reported carbapenemases (KPC, OXA-48-like, VIM, IMP, and NDM). Direct testing of positive blood cultures could reduce time to detection. This study aims to validate and report on the diagnostic yield of a novel method for carbapenemase detection in positive blood culture vials using NG-Test<sup>®</sup> CARBA 5.</p><p><strong>Methods: </strong>We implemented an investigator-developed method for the direct testing of positive blood cultures using NG-Test<sup>®</sup> CARBA 5. We compared results between genotypic, phenotypic, and direct NG-Test<sup>®</sup> CARBA 5 in blood.</p><p><strong>Results: </strong>A total of 32 isolates were tested (21 Enterobacterales and 11 <i>Pseudomonas aeruginosa</i>). Genotypic testing detected 23 carbapenemases. When comparing the results of NG-Test<sup>®</sup> CARBA 5 in blood with genotypic testing, agreement was observed in 31/32 (97%) tests. The sensitivity, specificity, positive predictive value, and negative predictive value of the NG-Test<sup>®</sup> CARBA 5 in blood were 93%, 100%, 100%, and 94%, respectively.</p><p><strong>Conclusions: </strong>Our method using NG-Test<sup>®</sup> CARBA 5 directly in blood culture samples presented an excellent diagnostic yield when compared to genotypic profiling and permits an accurate detection of carbapenemases.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"13 11","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591157/pdf/","citationCount":"0","resultStr":"{\"title\":\"Rapid Detection of Carbapenemases Using NG-Test<sup>®</sup> CARBA 5 in Positive Blood Cultures: A Diagnostic Test Study.\",\"authors\":\"Diana Munguia-Ramos, Luis Fernando Xancal-Salvador, Verónica Esteban-Kenel, Narciso Ortiz-Conchi, Ricardo Antonio Jaimes-Aquino, Miguel Mendoza-Rojas, Axel Cervantes-Sánchez, Steven Méndez-Ramos, Hector Orlando Rivera-Villegas, Sandra Rajme-Lopez, Karla Maria Tamez-Torres, Carla Marina Roman-Montes, Areli Martínez-Gamboa, Miriam Bobadilla Del-Valle, Jose Sifuentes-Osornio, Alfredo Ponce-de-Leon, Maria Fernanda Gonzalez-Lara, Bernardo Alfonso Martinez-Guerra\",\"doi\":\"10.3390/antibiotics13111105\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Infections due to carbapenem-resistant Gram-negative bacteria are emerging as an important challenge in health-care settings and a growing concern worldwide. Lateral flow immunoassay NG-Test<sup>®</sup> CARBA 5 can detect the five most reported carbapenemases (KPC, OXA-48-like, VIM, IMP, and NDM). Direct testing of positive blood cultures could reduce time to detection. This study aims to validate and report on the diagnostic yield of a novel method for carbapenemase detection in positive blood culture vials using NG-Test<sup>®</sup> CARBA 5.</p><p><strong>Methods: </strong>We implemented an investigator-developed method for the direct testing of positive blood cultures using NG-Test<sup>®</sup> CARBA 5. We compared results between genotypic, phenotypic, and direct NG-Test<sup>®</sup> CARBA 5 in blood.</p><p><strong>Results: </strong>A total of 32 isolates were tested (21 Enterobacterales and 11 <i>Pseudomonas aeruginosa</i>). Genotypic testing detected 23 carbapenemases. When comparing the results of NG-Test<sup>®</sup> CARBA 5 in blood with genotypic testing, agreement was observed in 31/32 (97%) tests. The sensitivity, specificity, positive predictive value, and negative predictive value of the NG-Test<sup>®</sup> CARBA 5 in blood were 93%, 100%, 100%, and 94%, respectively.</p><p><strong>Conclusions: </strong>Our method using NG-Test<sup>®</sup> CARBA 5 directly in blood culture samples presented an excellent diagnostic yield when compared to genotypic profiling and permits an accurate detection of carbapenemases.</p>\",\"PeriodicalId\":54246,\"journal\":{\"name\":\"Antibiotics-Basel\",\"volume\":\"13 11\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591157/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antibiotics-Basel\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3390/antibiotics13111105\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics13111105","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Rapid Detection of Carbapenemases Using NG-Test® CARBA 5 in Positive Blood Cultures: A Diagnostic Test Study.
Background: Infections due to carbapenem-resistant Gram-negative bacteria are emerging as an important challenge in health-care settings and a growing concern worldwide. Lateral flow immunoassay NG-Test® CARBA 5 can detect the five most reported carbapenemases (KPC, OXA-48-like, VIM, IMP, and NDM). Direct testing of positive blood cultures could reduce time to detection. This study aims to validate and report on the diagnostic yield of a novel method for carbapenemase detection in positive blood culture vials using NG-Test® CARBA 5.
Methods: We implemented an investigator-developed method for the direct testing of positive blood cultures using NG-Test® CARBA 5. We compared results between genotypic, phenotypic, and direct NG-Test® CARBA 5 in blood.
Results: A total of 32 isolates were tested (21 Enterobacterales and 11 Pseudomonas aeruginosa). Genotypic testing detected 23 carbapenemases. When comparing the results of NG-Test® CARBA 5 in blood with genotypic testing, agreement was observed in 31/32 (97%) tests. The sensitivity, specificity, positive predictive value, and negative predictive value of the NG-Test® CARBA 5 in blood were 93%, 100%, 100%, and 94%, respectively.
Conclusions: Our method using NG-Test® CARBA 5 directly in blood culture samples presented an excellent diagnostic yield when compared to genotypic profiling and permits an accurate detection of carbapenemases.
Antibiotics-BaselPharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍：
Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
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