miRNA 在代谢功能障碍相关性脂肪肝患者乙肝疫苗反应中的预测作用

IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2024-11-20 DOI:10.3390/v16111799
Gamze Guney Eskiler, Oguz Karabay, Mukaddes Tozlu, Ayhan Aydin, Kaan Furkan Hamarat, Umut Alkurt, Asuman Deveci Ozkan, Yasemin Gunduz
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引用次数: 0

摘要

(1) 背景:代谢功能障碍相关性脂肪性肝病(MASLD)是最常见的慢性肝病。虽然已有研究评估了乙型肝炎病毒相关疾病中微小 RNA(miRNA)表达水平的变化,但还没有研究评估了 miRNA 在 MASLD 患者 HBV 疫苗应答中的作用。我们的目的是根据 HBV 疫苗反应确定 MASLD 患者的 miRNA 表达谱。(2)方法:共纳入 100 名 MASLD 患者和 100 名对照组,在接种三剂 HBV 疫苗后测量抗 HBs 水平。采集血样后,通过 RT-PCR 分析 22 种不同 miRNA 的表达谱,并进一步验证所有研究组中潜在 miRNA 表达水平的变化。(3)结果:与对照组相比,MASLD 患者的 miR-146a 表达水平明显升高。此外,AntiHBs(-)健康人的 miR-99a 和 miR-640 表达水平也明显升高。(4):结论:miR-146a 可作为 MASLD 患者的诊断标志物。此外,miR-99a 和 miR-640 的表达水平可预测乙肝疫苗反应。然而,要验证 miRNA 在更多患者中的生物标记潜力,还需要进行验证研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Predictive Role of miRNAs in Hepatitis B Vaccine Response of Metabolic Dysfunction-Associated Steatotic Liver Disease Patients.

(1) Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common chronic liver disease. Although the changes in the expression levels of microRNAs (miRNAs) in hepatitis B virus-related diseases have been evaluated, no study has evaluated the role of miRNAs in HBV vaccine response in MASLD patients. We aimed to determine the miRNA expression profile in MASLD patients according to HBV vaccine response. (2) Methods: Overall, 100 MASLD patients and 100 controls were included, and anti-HBs levels were measured after three doses of HBV vaccine administration. After collecting blood samples, 22 different miRNA expression profiles were analyzed by RT-PCR analysis, and changes in the expression levels of potential miRNAs were further verified in all study groups. (3) Results: The miR-146a expression level considerably increased in MASLD patients compared to the control group. Furthermore, miR-99a and miR-640 expression levels significantly increased in AntiHBs (-) healthy individuals. (4): Conclusions: miR-146a could be used as the diagnostic marker in MASLD patients. Furthermore, the miR-99a and miR-640 expression levels could predict hepatitis B vaccine response. However, validation studies are required to verify the biomarker potential of miRNAs within a more significant number of patients.

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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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