牛伽马疱疹病毒 6 在自然宿主中的趋向性。

IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2024-11-03 DOI:10.3390/v16111730
Rosalie Fabian, Giuliana Rosato, James P Stewart, Anja Kipar
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引用次数: 0

摘要

牛γ疱疹病毒 6(BoHV-6)是欧洲牛的地方病,发病率很高。有证据表明,该病毒是一种共生病毒,与疾病过程无关。众所周知,其他γ疱疹病毒的靶细胞谱相当特殊,一般包括 B 细胞和呼吸道上皮细胞,至少在感染的早期阶段是如此。在之前的一项研究中,我们通过定量 PCR 检测了肺部、支气管淋巴结、脾脏和舌头中 BoHV-6 的 gB 基因序列,结果显示这些组织中的细胞是靶细胞。在本研究中,通过 RNA 原位杂交检测 BoHV-6 ORF73,对来自 10 头牛的相同组织的福尔马林固定石蜡包埋样本进行了检测,这些牛的 BoHV-6 总拷贝数较高。结果显示,病毒 ORF73 的转录极其有限。仅在支气管淋巴结淋巴滤泡中的单个淋巴细胞以及肺部极少数肺泡上皮细胞和间质细胞中检测到信号,组织中没有任何病理变化的证据。在脾脏或舌头的口腔粘膜中未检测到信号。这些结果与之前对其他γ疱疹病毒、鼠疱疹病毒-68、卵疱疹病毒-2和/或爱泼斯坦-巴尔病毒的研究结果一致。这些结果进一步证明,BoHV-6 对宿主没有任何影响,而且确实可以作为牛的共生病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bovine Gammaherpesvirus 6 Tropism in the Natural Host.

Bovine gammaherpesvirus 6 (BoHV-6) is endemic in cattle in Europe, with a high prevalence. There is evidence that the virus is a commensal and not associated with disease processes. For other gammaherpesviruses, it is known that they have a rather specific target cell spectrum, generally including B cells and, at least in the early phase of infection, the epithelium of the respiratory tract. In a previous study we detected BoHV-6 by quantitative PCR for the gB gene sequence of BoHV-6 in lung, bronchial lymph nodes, spleen and tongue with variable loads, suggesting cells in these tissues as target cells. In the present study, formalin-fixed, paraffin embedded samples of the same tissues from 10 cattle, with high overall BoHV-6 copy numbers, were examined by RNA in situ hybridization for BoHV-6 ORF73. This revealed extremely limited viral ORF73 transcription. A signal was only detected in individual lymphocytes within lymphatic follicles in bronchial lymph nodes, and within very rare alveolar epithelial cells and interstitial cells in the lungs, without any evidence of pathological changes in the tissues. No signal was detected in the spleen or in the oral mucosa of the tongue. The results are consistent with previous findings with other gammaherpesviruses, murine herpesvirus-68, ovine herpesvirus-2 and/or Epstein-Barr virus. They provide further evidence that BoHV-6 is without any consequence to the host and can indeed represent a commensal in cattle.

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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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