半灌木冲天香精油和球桉树精油对突尼斯患病动物中耐药大肠埃希氏菌和大肠杆菌的功效

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Sana Dhaouadi, Salma Kloula Ben Ghorbel, Wafa Bouglita, Soufiene Chaari, Wissal Dhifi, Rim Khrouf, Ameur Cherif, Ramzi Boubaker Elandoulsi
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引用次数: 0

摘要

本研究旨在探讨半灌木(CSEO)和球桉树(EGEO)精油对从突尼斯患病食用动物中分离出的耐大肠杆菌和耐甲氧西林球孢霉烯菌的抗菌活性。使用气相色谱-质谱法(GC-MS)分析了两种精油的化学成分。抗菌活性是通过盘扩散和微量稀释法测定的,而分数抑菌浓度指数(FICI)则用于测量 CSEO 和可乐定的协同作用。CSEO 的主要成分为 α-蒎烯(59.03%)、δ-3 Carene(21.48%)、D-柠檬烯(5.62%)和 α-��烯(3.08%),而 EGEO 含有桉叶油醇(52.1%)、α-蒎烯(26.51%)、γ-萜品烯(4.81%)和 D-柠檬烯(3.54%)。CSEO 和 EGEO 对大肠杆菌和 M. sciuri 的抑制区直径分别为 6 至 29 毫米和 9.3 至 28.6 毫米。CSEO 对恙虫病菌的最小抑菌浓度(MIC)为 4 至 512 µg/mL,EGEO 为 2 至 426.66 µg/mL。对于大肠杆菌,CSEO 的 MIC 在 170.66 至 512 µg/mL 之间,EGEO 的 MIC 在 53.33 至 512 µg/mL 之间。棋盘试验表明,CSEO 和可乐定对两种耐可乐定的大肠杆菌分离物具有协同作用,其 FICI 比单个化合物的 MIC 低 3 到 32 倍。这项研究强调了 CSEO 和 EGEO 的精油对突尼斯动物体内耐抗生素的 M. sciuri 和大肠杆菌菌株的功效,并提供了 CSEO 和大肠菌素对临床相关的耐大肠菌素大肠杆菌分离物具有协同作用的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The potency of Cupressus sempervirens and Eucalyptus globulus Essential Oils Against Antibiotic-Resistant Escherichia coli and Mammaliicoccus sciuri from Diseased Animals in Tunisia.

This study aimed to investigate the antibacterial activity of essential oils from Cupressus sempervirens (CSEO) and Eucalyptus globulus (EGEO) against clinical isolates of colistin-resistant E. coli and methicillin-resistant M. sciuri recovered from diseased food-producing animals in Tunisia. The chemical compositions of both essential oils were analyzed using Gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was determined through disk diffusion and microdilution assays, while the fractional inhibitory concentration index (FICI) was used to measure the synergistic effect of CSEO and colistin. The major constituents of CSEO were α-pinene (59.03%), δ-3 Carene (21.48%), D-limonene (5.62%) and α-thujene (3.08%), while EGEO contained eucalyptol (52.1%), α-pinene (26.51%), γ-terpinene (4.81%) and D-limonene (3.54%). The inhibition zone diameters (IZDs) against E. coli and M. sciuri ranged from 6 to 29 mm for CSEO and 9.3 to 28.6 mm for EGEO. Minimum inhibitory concentrations (MICs) showed a range of 4 to 512 µg/mL for CSEO against M. sciuri and 2 to 426.66 µg/mL for EGEO. For E. coli, MICs ranged from 170.66 to 512 µg/mL for CSEO and 53.33 to 512 µg/mL for EGEO. The checkerboard test demonstrated a synergistic effect between CSEO and colistin against two colistin-resistant E. coli isolates, with FICI 3- to 32-fold lower than the MICs of the individual compounds. This study highlights the potency of essential oils from CSEO and EGEO against antibiotic-resistant M. sciuri and E. coli strains from animals in Tunisia and provides evidence of the synergistic effect between CSEO and colistin against clinically relevant colistin-resistant E. coli isolates.

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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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