Francisco Moreno, Lucía Méndez, Ingrid Fernández, Bernat Miralles-Pérez, Montserrat Giralt, Marta Romeu, Sara Ramos-Romero, Josep Lluís Torres, Isabel Medina
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The results were compared with those induced by oils with a lower degree of fat unsaturation (fish oil containing 25% DHA and 25% eicosapentaenoic acid, soybean oil containing 50% linoleic acid and coconut oil containing 90% saturated fat). The results demonstrated that fish oil containing 80% DHA significantly increased the ω3/ω6 ratio in both the cortex and cerebellum while stimulating antioxidant defense by enhancing the reduced glutathione amount and decreasing the carbonylation of specific proteins, mainly those involved in glycolysis and neurotransmission. The majority of sensitive proteins in both brain regions followed this carbonylation trend (in decreasing order): soybean > EPA/DHA 1:1 > coconut > 80% DHA. The results also indicated that the cerebellum is more responsive than the cortex to changes in the cellular redox environment induced by varying degrees of fat unsaturation. 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引用次数: 0
摘要
ω-3多不饱和脂肪酸(PUFA)对大脑结构和功能至关重要,尤其是二十二碳六烯酸(DHA)。然而,由于 DHA 的不饱和度较高,过量的 DHA 可能会增加脂质过氧化,尤其是在大脑等极易受到氧化应激影响的组织中。因此,本研究评估了雄性 Sprague Dawley 大鼠膳食中补充 10 周含有 80% DHA 的鱼油对氧化应激以及大脑皮层和小脑中羰基化蛋白质组调节的影响。结果与脂肪不饱和度较低的油类(含 25% DHA 和 25% 二十碳五烯酸的鱼油、含 50% 亚油酸的大豆油和含 90% 饱和脂肪的椰子油)所诱导的结果进行了比较。结果表明,含 80% DHA 的鱼油能显著提高大脑皮层和小脑中的ω3/ω6 比率,同时通过提高还原型谷胱甘肽的含量和减少特定蛋白质(主要是参与糖酵解和神经传递的蛋白质)的羰基化来刺激抗氧化防御。两个脑区的大多数敏感蛋白质都遵循这种羰基化趋势(按递减顺序排列):大豆 > EPA/DHA 1:1 > 椰子油 > 80% DHA。研究结果还表明,小脑比大脑皮层对不同程度的脂肪不饱和引起的细胞氧化还原环境变化反应更敏感。总之,在健康条件下,与 DHA 含量较低、脂肪酸不饱和度较低的鱼油相比,通过膳食补充含有高含量 DHA 的鱼油能使大脑对潜在的氧化损伤更有抵抗力。
Influence of the Degree of Unsaturation in Fish Oil Supplements on Oxidative Stress and Protein Carbonylation in the Cerebral Cortex and Cerebellum of Healthy Rats.
ω-3 polyunsaturated fatty acids (PUFAs) are crucial for brain structure and function, especially docosahexaenoic acid (DHA). However, an excess of DHA may increase lipid peroxidation due to its high degree of unsaturation, particularly in tissues highly susceptible to oxidative stress, such as the brain. Therefore, this study evaluated the effects of 10 weeks of dietary supplementation with fish oil containing 80% DHA on oxidative stress and the modulation of the carbonylated proteome in both the cerebral cortex and cerebellum of male Sprague Dawley rats. The results were compared with those induced by oils with a lower degree of fat unsaturation (fish oil containing 25% DHA and 25% eicosapentaenoic acid, soybean oil containing 50% linoleic acid and coconut oil containing 90% saturated fat). The results demonstrated that fish oil containing 80% DHA significantly increased the ω3/ω6 ratio in both the cortex and cerebellum while stimulating antioxidant defense by enhancing the reduced glutathione amount and decreasing the carbonylation of specific proteins, mainly those involved in glycolysis and neurotransmission. The majority of sensitive proteins in both brain regions followed this carbonylation trend (in decreasing order): soybean > EPA/DHA 1:1 > coconut > 80% DHA. The results also indicated that the cerebellum is more responsive than the cortex to changes in the cellular redox environment induced by varying degrees of fat unsaturation. In conclusion, under healthy conditions, dietary supplementation with fish oils containing high DHA levels makes the brain more resilient to potential oxidative insults compared to oils with lower DHA content and a lower degree of fatty acid unsaturation.
AntioxidantsBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍:
Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.