Linxin Cao, Wenhui Chen, Wenyuan Kang, Chunyang Lei, Zhou Nie
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引用次数: 0
摘要
CRISPR-Cas 系统的精确目标识别和核酸酶介导的有效信号放大能力在生物传感领域引起了相当大的研究兴趣。在对其结构和生化机制的深入研究的指导下，研究人员致力于设计具有刺激响应功能的 CRISPR-Cas 系统的关键生物组件。通过结合蛋白质/核酸工程技术，人们建立了多种有条件的 CRISPR-Cas 系统，这些系统的活性取决于目标触发器的存在，用于高效检测各种类型的非核酸分析物。在这篇综述中，我们总结了最近在工程化 Cas 蛋白、引导 RNA 和底物核酸方面的研究进展，这些工程化的 Cas 蛋白、引导 RNA 和底物核酸具有目标分析物响应能力，可用于多种生物传感应用。此外，我们还讨论了刺激响应型 CRISPR-Cas 系统在多功能生物传感中面临的挑战和未来的可能性。
Engineering stimuli-responsive CRISPR-Cas systems for versatile biosensing.
The precise target recognition and nuclease-mediated effective signal amplification capacities of CRISPR-Cas systems have attracted considerable research interest within the biosensing field. Guided by insights into their structural and biochemical mechanisms, researchers have endeavored to engineer the key biocomponents of CRISPR-Cas systems with stimulus-responsive functionalities. By the incorporation of protein/nucleic acid engineering techniques, a variety of conditional CRISPR-Cas systems whose activities depend on the presence of target triggers have been established for the efficient detection of diverse types of non-nucleic acid analytes. In this review, we summarized recent research progress in engineering Cas proteins, guide RNA, and substrate nucleic acids to possess target analyte-responsive abilities for diverse biosensing applications. Furthermore, we also discussed the challenges and future possibilities of the stimulus-responsive CRISPR-Cas systems in versatile biosensing.
期刊介绍：
Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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