TaGPAT6 通过合成角质素和单宁单体来形成扩散屏障,从而增强小麦的耐盐性。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Wenlong Wang, Menghan Chi, Shupeng Liu, Ying Zhang, Jiawang Song, Guangmin Xia, Shuwei Liu
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引用次数: 0

摘要

植物耐受高盐度的机制之一是沉积角质素和单宁,以形成限制离子流入的凋落物屏障。然而,盐胁迫下屏障形成的机制尚不清楚。在此,我们对甘油-3-磷酸酰基转移酶(GPAT)家族基因 TaGPAT6 进行了鉴定,该基因编码的蛋白质参与了小麦(Triticum aestivum)中角质素和单宁的生物合成,以形成凋落物屏障。TaGPAT6 具有酰基转移酶和磷酸酶活性,负责合成角质素和单宁酸的前体--sn-2-单酰基甘油(sn-2 MAG)。过表达 TaGPAT6 可促进角质素和单宁在种皮和根尖细胞外层的沉积,并通过减少细胞内钠离子的积累来提高耐盐性。相比之下,TaGPAT6 基因敲除突变体对盐胁迫的敏感性增加,原因是角质和小胶皮沉积减少,钠离子积累增加。酵母一杂交和电泳迁移测定确定了 TaABI5 是 TaGPAT6 的上游调节因子。TaABI5 基因敲除突变体显示 TaGPAT6 的表达受到抑制,种皮中屏障的形成减少。这些结果表明,TaGPAT6 参与了角质素和单宁的生物合成以及由此形成的凋落物屏障,从而增强了小麦的耐盐性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TaGPAT6 enhances salt tolerance in wheat by synthesizing cutin and suberin monomers to form a diffusion barrier.

One mechanism plants use to tolerate high salinity is the deposition of cutin and suberin to form apoplastic barriers that limit the influx of ions. However, the mechanism underlying barrier formation under salt stress is unclear. Here, we characterized the glycerol-3-phosphate acyltransferase (GPAT) family gene TaGPAT6, encoding a protein involved in cutin and suberin biosynthesis for apoplastic barrier formation in wheat (Triticum aestivum). TaGPAT6 has both acyltransferase and phosphatase activities, which are responsible for the synthesis of sn-2-monoacylglycerol (sn-2 MAG), the precursor of cutin and suberin. Overexpressing TaGPAT6 promoted the deposition of cutin and suberin in the seed coat and the outside layers of root tip cells and enhanced salt tolerance by reducing sodium ion accumulation within cells. By contrast, TaGPAT6 knockout mutants showed increased sensitivity to salt stress due to reduced cutin and suberin deposition and enhanced sodium ion accumulation. Yeast-one-hybrid and electrophoretic mobility shift assays identified TaABI5 as the upstream regulator of TaGPAT6. TaABI5 knockout mutants showed suppressed expression of TaGPAT6 and decreased barrier formation in the seed coat. These results indicate that TaGPAT6 is involved in cutin and suberin biosynthesis and the resulting formation of an apoplastic barrier that enhances salt tolerance in wheat.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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