How Heatwaves Impact Microalgae in the Presence of Environmentally Relevant PFAS Concentration: Metabolic Shifts and Challenges Posed

Per- and polyfluoroalkyl substances (PFAS) are widely distributed in the aquatic environment. While increasing studies have investigated the effects of specific PFAS exposure on microalgae, the impact of environmentally relevant PFAS concentrations, particularly during extreme weather events like heatwaves, remains unclear. For Microcystis aeruginosa, a cyanobacteria causing harmful algal blooms, PFAS exposure promoted growth and photosynthesis by accelerating the TCA cycle, intensifying carbon/nitrogen and nucleotide metabolism, and enhancing antioxidant expression. Moreover, although heatwave exposure alone adversely affected algal growth, co-exposure to PFAS and heatwaves paradoxically enhanced algal growth. This co-exposure also enhanced the expression of photosynthetic pigments and metabolites involved in alanine, aspartate and glutamate metabolism, as well as arginine and proline metabolism (compared to PFAS exposure alone). Nevertheless, co-exposure intensified oxidative stress, leading to differential expression of antioxidants, which may consequently affect the synthesis of membrane lipids. In addition, PFAS adsorption and uptake are primarily influenced by the varying strengths of PFAS molecules in binding with proteins and notably boosted by heatwaves. This study highlights the role of diverse PFAS in microalgae blooms and the influence of heatwave events on pollutant responses, providing scientific foundations for aquatic ecosystem protection against climate and pollution challenges.