利用 UPLC-ESI-MS/MS 对拭子样本中的五种抗肿瘤药物进行定量：方法开发与验证

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-27 DOI:10.1016/j.aca.2024.343492

Eline Verscheure , Matteo Creta , Katrien Poels , Jeroen Vanoirbeek , Manosij Ghosh , Lode Godderis

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引用次数: 0

摘要

背景抗肿瘤药物是用于治疗癌症的危险药物，因此可能存在于工作场所（如医院），也可能存在于家庭环境中（如在家中治疗）。鉴于其对健康的严重影响，定期监测环境污染非常重要。由于目前还没有一种方法可以同时定量检测环磷酰胺、依托泊苷、丝裂霉素 C、伊马替尼和 5-氟尿嘧啶，因此我们开发并验证了一种同时定量检测拭子样本中多种抗肿瘤药物的方法。采用超高效液相色谱-电喷雾串联质谱法检测化合物。测定了选择性、线性、灵敏度、精密度和准确度。LLOQ 为 0.3 ng/swab（环磷酰胺、丝裂霉素 C）、1 ng/swab（依托泊苷）和 5 ng/swab（伊马替尼、5-氟尿嘧啶）。溢出后，可使用所开发的方法测量拭子中可量化的抗肿瘤药物浓度。意义开发出了两种可安全收集、快速制备和灵敏定量的方法，可用于实地测量环境污染。一种用于同时定量环磷酰胺、依托泊苷、丝裂霉素 C 和伊马替尼，一种用于定量 5-氟尿嘧啶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Quantification of five antineoplastic agents in swab samples using UPLC-ESI-MS/MS: Method development and validation

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Quantification of five antineoplastic agents in swab samples using UPLC-ESI-MS/MS: Method development and validation

Background

Antineoplastic agents are hazardous drugs used in cancer treatment and consequently can be present at the workplace (e.g. hospital), but also in a home-setting in case of treatment at home. Given the serious health effects, it is important to monitor environmental contamination on a regular base. As currently, no method allows for simultaneous quantification of cyclophosphamide, etoposide, mitomycin C, imatinib and 5-fluorouracil, we developed and validated a method for simultaneous quantification of multiple antineoplastic agents in swab samples.

Results

Sample collection was optimised and samples were extracted using solely an extraction solvent (water or water/acetonitrile), shaking and sonicating. Ultra performance liquid chromatography coupled to tandem mass spectrometry with electrospray ionisation was used for detection of the compounds. Selectivity, linearity, sensitivity, precision and accuracy were determined. The LLOQs were 0.3 ng/swab (cyclophosphamide, mitomycin C), 1 ng/swab (etoposide) and 5 ng/swab (imatinib, 5-fluorouracil). After a spill, quantifiable concentrations of antineoplastic agents could be measured in swabs using the developed methods. After cleaning, all concentrations fell below the LLOQ.

Significance

Two methods allowing for safe collection, rapid preparation and sensitive quantification were developed and can be applied in the field to measure environmental contamination. One for simultaneous quantification of cyclophosphamide, etoposide, mitomycin C and imatinib, one for quantification of 5-fluorouracil.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.