灭活绚丽弧菌的免疫刺激促进了巨牡蛎幼体的发育和抵抗力。

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2024-11-23 DOI:10.1016/j.fsi.2024.110041

Junlei Cheng , Weilin Wang , Tianqi Yu , Xiaoxu Zhou , Xingye Lian , Xuemei Cheng , Lingling Wang , Linsheng Song

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引用次数: 0

摘要

处于胚胎发育阶段的牡蛎幼体在孵化过程中死亡率一直居高不下，严重阻碍了牡蛎育苗产业的可持续发展。本研究探讨了用灭活的绚丽弧菌对处于胚胎发育中期（受精后 10 d，dpf）的牡蛎幼体的免疫保护和发育效应。结果表明，用灭活的白弧菌进行免疫前刺激后，牡蛎幼体的免疫系统被激活。免疫识别受体（CTL-3、整合素β-1、TLR4）、NF-kB信号转导元件（IKK）、效应分子（IL17-5、Defh2、HSP70）的表达明显上调，抗氧化酶（超氧化物歧化酶和过氧化氢酶）、水解酶（溶菌酶）的活性也显著增加。受刺激的褐飞虱幼虫蛋白质具有明显的凝集和抑制白芨生长的特性。用白斑褐飞虱幼虫（18 dpf）或幼虫期（21 dpf）的活体挑战白斑褐飞虱，刺激组的死亡率比对照组低得多。同时，在免疫刺激组中，上述免疫相关基因的表达和抗氧化酶的活性都迅速上调。此外，灭活的白弧菌刺激后，鳗鲡幼体的壳高、壳长明显增加，发育速度加快，沉降率提高。总之，灭活的白弧菌刺激牡蛎脐带幼体可激活其免疫系统，增强其对白弧菌感染的抵抗力，直至足龄阶段，并促进其后续生长发育。所有这些结果为解决牡蛎育苗产业中幼体抗病性差、死亡率高的问题提供了理论依据。

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Immune stimulation by inactivated Vibrio splendidus promotes the development and resistance of oyster Crassostrea gigas larvae

The oyster Crassostrea gigas larvae in embryonic development stage have been suffering high mortality rate during hatching, which have seriously hindered the sustainable development of oyster seedling industry. This study explored the immune protection and developmental effects of priming with inactivated Vibrio splendidus in oyster larvae at middle umbo stage (10 d post fertilization, dpf). The results showed that the immune system of umbo larvae was activated after pre-immune stimulation with inactivated V. splendidus. The expressions of immune recognition receptors (CTL-3, Integrin β-1, TLR4), NF-kB signaling component (IKK), effector molecules (IL17-5, Defh2, HSP70) were significantly up-regulated, and the activities of antioxidant enzymes (superoxide dismutase and catalase), hydrolytic enzyme (lysozyme) also increased significantly. The proteins from the stimulated umbo larvae have obvious characteristics of agglutination and inhibition of V. splendidus growth. When the larvae at late umbo (18 dpf) or pediveliger stage (21 dpf) were challenged by live V. splendidus, much lower death rate was observed in the stimulation group compared to the control group. Simultaneously, the expressions of above immune related genes and the activities of antioxidant enzymes were all rapidly up-regulated in pediveliger larvae of immune stimulated group. Moreover, the significantly increased shell height and shell length as well as accelerated development rate, and higher settlement rate were revealed after the umbo larvae are stimulated by inactivated Vibrio splendidus. In summary, inactivated V. splendidus stimulation in oyster umbo larvae could activate their immune system, enhance their resistance against V. splendidus infection till to pediveliger stage, and promote their following growth and development. All the results provided a theoretical basis for solving poor disease resistance and high mortality of larvae in oyster seedling industry.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.