分析宫颈癌前病变和宫颈炎中孤儿核受体 NR4A1、NR4A2 和 NR4A3 的基因表达谱

IF 1.5 Q3 OBSTETRICS & GYNECOLOGY

European Journal of Obstetrics and Gynecology and Reproductive Biology: X Pub Date : 2024-11-13 DOI:10.1016/j.eurox.2024.100355

Rosa P. Cruz-Nieves , Gladys E. Ramírez-Rosales , Javier González-Ramírez , Fausto Sánchez-Muñoz , Armando Ruiz-Hernández

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引用次数: 0

摘要

本研究的主要目的是评估核孤儿受体在子宫颈癌（UCC）发病过程中的表达。宫颈上皮发育不良的主要原因是人乳头状瘤病毒的存在，它可导致宫颈上皮内瘤变 I（CIN I）、高级别病变（CIN II 和 CIN III），最后发展为浸润性癌。尽管有各种治疗方法和疫苗，但死亡率仍然很高。有证据表明，一组被称为 "孤儿 "的核受体参与了包括癌症在内的各种疾病的发展。研究设计采用实时聚合酶链式反应（RT-PCR）方法测定了在不列颠哥伦比亚省墨西卡利市肿瘤内科专科（UNEME）就诊的 45 名患者通过阴道镜检查获得的样本中孤儿受体 NR4A1、NR4A2 和 NR4A3 的表达水平。我们的研究结果表明，孤儿受体以特定的方式表达，这取决于癌前病变的程度。NR4A1 在宫颈炎中过表达（p < 0.05）。我们的数据首次表明，核受体可能参与了侵袭性 UCC 发生前的各个阶段。

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Analysing the gene expression profiles of the orphan nuclear receptors NR4A1, NR4A2 and NR4A3 in premalignant lesions of the cervix and cervicitis

Objective

The main objective of the present study was to evaluate the expression of nuclear orphan receptors in the development of Uterine Cervical Cancer (UCC). The principal cause of dysplastic changes in cervical epithelium is the presence of the human papilloma virus leading to the development of cervical intraepithelial neoplasia I (CIN I), high-grade lesions (CIN II and CIN III) and, finally, invasive cancer. Despite the existence of various treatments and vaccines, there is still a high mortality rate. There is evidence of the participation of a group of nuclear receptors called orphans in the development of various diseases, including cancer.

Study design

The expression levels of the orphan receptors NR4A1, NR4A2, and NR4A3 were measured using real-time polymerase chain reaction (RT-PCR) in samples obtained through colposcopy from forty-five patients who attended the Medical Oncology Specialties Unit (UNEME) in Mexicali, B.C.

Results

Forty-five cervical biopsy results were obtained, indicating cervicitis, CIN I, or CIN III, none of them CIN II. Our results showed that orphan receptors expressed in a specific manner depending on the degree of premalignant lesions. NR4A1 overexpressed in cervicitis (p < 0.05). NR4A3 was significantly expressed in CIN I (p < 0.05) and NR4A2 was expressed in both cervicitis and CIN III (p > 0.05).