癌症相关成纤维细胞源性细胞外囊泡中的 SNHG12 可诱导巨噬细胞-成纤维细胞转化。

IF 3 4区医学 Q2 GENETICS & HEREDITY

Epigenomics Pub Date : 2024-12-01 Epub Date: 2024-11-20 DOI:10.1080/17501911.2024.2430166

Tao Wu, Li Zeng, Chao Peng, Zheng Zhao, Weihao Mu, Song Wang, Deli Tan

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引用次数: 0

摘要

目的：研究非小细胞肺癌（NSCLC）中癌相关成纤维细胞（CAFs）产生的细胞外囊泡（EVs）中lncRNA SNHG12诱导巨噬细胞-肌成纤维细胞转化（MMT）的机制：方法：从人类NSCLC组织和邻近癌组织（n = 3）中分离出CAFs EVs，并对其形态和颗粒大小进行评估。检测了不同表型的巨噬细胞和MMT细胞，并验证了lncRNA SNHG12、miR-181a-5p和Smad3的结合关系：结果：来源于CAFs-EVs的LncRNA SNHG12促进了M2巨噬细胞向MMT的转化。此外，lncRNA-SNHG12还增加了Smad3的表达，而Smad3通过miR-181a-5p的海绵作用在MMT中显著上调：结论：来自CAFs-EV的LncRNA SNHG12可诱导NSCLC中的MMT。

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SNHG12 in cancer-associated fibroblast-derived extracellular vesicle induces macrophage-myofibroblast transition.

Aims: To investigate mechanism of lncRNA SNHG12 induced macrophage-myofibroblast transition (MMT) in cancer-associated fibroblasts (CAFs)-derived extracellular vesicles (EVs) in non-small cell lung cancer (NSCLC).

Method: CAFs EVs were isolated from human NSCLC tissue and adjacent cancerous tissue (n = 3), and their morphology and particle size were evaluated. Macrophages and MMT cells with different phenotypes were detected, and the binding relationship of lncRNA SNHG12, miR-181a-5p, and Smad3 was verified.

Result: LncRNA SNHG12 derived from CAFs-EVs promoted the transformation of M2 macrophages into MMT. In addition, lncRNA-SNHG12 increased the expression of Smad3 which was significantly upregulated in MMT through sponge of miR-181a-5p.

Conclusion: LncRNA SNHG12 derived from CAFs-EV induced MMT in NSCLC.

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来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.