Ligia Maria Salvo, Mara Elisa Gazino Joineau, Magda Regina Santiago
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引用次数: 0
摘要
农药被稀释在称为溶剂的产品中，用于田间喷洒和细胞活力研究。本研究旨在确定农药溶剂是否会改变硫丹的毒性和 Vero 细胞的活力。因此，通过中性红掺入技术和细胞生长，对农药溶液中常用的一些稀释剂的细胞毒性进行了评估。将硫丹溶于向日葵（Helianthus annus，Linnaeus）油、玉米（Zea mays，Linnaeus）油、二甲基亚砜（DMSO）和吐温 80（浓度为 1 µg L-1）中，让 Vero 细胞接触 96 小时。此外，吐温 80 具有明显的细胞毒性作用（P
In vitro cytotoxicity assessment of different solvents used in pesticide dilution.
Pesticides are diluted in products called solvents for spraying fields and for cell viability studies. This study aimed to determine whether pesticide solvents can alter the toxicity of endosulfan and Vero cell viability. Thus, the cytotoxicity of some diluents commonly used in pesticide solutions was evaluated by the neutral red incorporation technique and cell growth. Vero cells were exposed to endosulfan dissolved in sunflower (Helianthus annus, Linnaeus) oil, corn (Zea mays, Linnaeus) oil, dimethylsulfoxide (DMSO), and Tween 80, at a concentration of 1 µg L-1 for a period of 96 h. The results showed that both DMSO and Tween 80 induced a significant increase in cytotoxicity compared to sunflower oil and corn oil. Moreover, Tween 80 had a significant cytotoxic effect (P < 0.05) when compared to DMSO. The solvent can alter the toxicity of endosulfan, decreasing Vero cell viability, as was the case with DMSO and Tween 80.
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