眼部用药的代谢组学特征:探索亚致死高剂量柚皮苷对 ARPE-19 细胞代谢的影响

IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE
Ozan Kaplan , Emine Koç , Seyhan Türk , Tuba Tüylü Küçükkılınç , Zeynep Göktaş , Mustafa Çelebier
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引用次数: 0

摘要

导言柚皮苷(NRG)是一种存在于柑橘类水果中的黄烷酮多酚,其对眼部疾病的潜在治疗作用已被越来越多的人所认识。本研究旨在通过代谢组学分析评估高剂量 NRG 对人类视网膜色素上皮细胞(ARPE-19)的影响。方法使用噻唑基溴化蓝四氮唑(MTT)检测法评估细胞活力,结果表明,在 3 到 100 μM 的浓度范围内,24 小时暴露期内细胞无毒性。高剂量(100 μM)NRG 无细胞毒性,因此被选作进一步研究的对象。采用液相色谱四极飞行时间质谱法(LC-Q-ToF-MS)进行色谱分析。结果代谢组学分析发现了微妙的代谢变化,对照组和试验组之间的代谢谱相似,这强调了 NRG 的细微影响。包括主成分分析(PCA)在内的多变量分析表明,对照组和测试组有明显的聚类,表明每组内的代谢调整是一致的。尽管保持了细胞的活力,但在 ARPE-19 细胞中发现了应激反应,表现为葡萄糖-6-磷酸水平的降低和嘧啶/嘌呤通路的改变。这凸显了利用代谢组学探索细胞反应的重要性,而非传统的存活率指标。 结论在更广泛的背景下,本研究为 NRG 与视网膜色素上皮之间的相互作用提供了数据。由于 NRG 可用于常规眼科应用,特别是作为眼药水使用,我们的研究结果表明,在选择剂量时应谨慎,既要考虑无刺激性,又要考虑微妙的新陈代谢变化。还需要进一步的研究来完善剂量策略,并全面评估 NRG 在眼部应用中的安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolomic signature in ocular dosing: Exploring the metabolic impacts of sublethal high-dose naringenin on ARPE-19 cells

Introduction

Naringenin (NRG), a flavanone polyphenol found in citrus fruits, has been increasingly recognized for its potential therapeutic effects in ocular disorders. This study aimed to assess the impact of high-dose NRG on human retinal pigment epithelial cells (ARPE-19) cells through metabolomic analysis.

Methods

Cell viability was evaluated using the thiazolyl blue tetrazolium bromide (MTT) assay, demonstrating non-cytotoxicity at concentrations ranging from 3 to 100 μM within a 24-h exposure period. High-dose (100 μM) NRG was selected for further investigation based on its non-cytotoxic nature. Chromatographic analyses were performed using Liquid Chromatography Quadropole Time-of-Flight Mass Spectrometry (LC-Q-ToF-MS).

Results

Metabolomic analysis revealed subtle metabolic changes, with similar profiles between control and test groups, emphasizing the nuanced effects of NRG. Multivariate analysis, including Principal Component Analysis (PCA), illustrated distinct clustering of control and test groups, indicating consistent metabolic adjustments within each group. Despite maintaining cell viability, stress responses were identified in ARPE-19 cells, as evidenced by reduced glucose-6-phosphate levels and alterations in pyrimidine/purine pathways. This highlights the importance of exploring cellular responses using metabolomics beyond traditional viability metrics.

Conclusion

In the broader context, this study contributes data on the interplay between NRG and retinal pigmented epithelial. As NRG is utilized in routine ocular applications, particularly as an eye drop, our findings suggest careful dosage selection, considering both non-irritability and subtle metabolic changes. Further research is needed to refine dosage strategies and comprehensively assess the safety profile of NRG in ocular applications.
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来源期刊
European Journal of Integrative Medicine
European Journal of Integrative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
4.70
自引率
4.00%
发文量
102
审稿时长
33 days
期刊介绍: The European Journal of Integrative Medicine (EuJIM) considers manuscripts from a wide range of complementary and integrative health care disciplines, with a particular focus on whole systems approaches, public health, self management and traditional medical systems. The journal strives to connect conventional medicine and evidence based complementary medicine. We encourage submissions reporting research with relevance for integrative clinical practice and interprofessional education. EuJIM aims to be of interest to both conventional and integrative audiences, including healthcare practitioners, researchers, health care organisations, educationalists, and all those who seek objective and critical information on integrative medicine. To achieve this aim EuJIM provides an innovative international and interdisciplinary platform linking researchers and clinicians. The journal focuses primarily on original research articles including systematic reviews, randomized controlled trials, other clinical studies, qualitative, observational and epidemiological studies. In addition we welcome short reviews, opinion articles and contributions relating to health services and policy, health economics and psychology.
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