具有大斯托克斯偏移的溶酶体靶向荧光探针，用于在体内和体外观察生物硫醇

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2024-11-07 DOI:10.1016/j.isci.2024.111334

Taotao Zhao , Tong Zhang , Zijun Tao , Zhe Zhou , Xiaofeng Xia , Zhengjun Wu , Feiyi Wang , Jun Ren , Erfei Wang

{"title":"具有大斯托克斯偏移的溶酶体靶向荧光探针，用于在体内和体外观察生物硫醇","authors":"Taotao Zhao , Tong Zhang , Zijun Tao , Zhe Zhou , Xiaofeng Xia , Zhengjun Wu , Feiyi Wang , Jun Ren , Erfei Wang","doi":"10.1016/j.isci.2024.111334","DOIUrl":null,"url":null,"abstract":"<div><div>Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols <em>in vivo</em> and <em>in vitro</em>. Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 12","pages":"Article 111334"},"PeriodicalIF":4.6000,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro\",\"authors\":\"Taotao Zhao , Tong Zhang , Zijun Tao , Zhe Zhou , Xiaofeng Xia , Zhengjun Wu , Feiyi Wang , Jun Ren , Erfei Wang\",\"doi\":\"10.1016/j.isci.2024.111334\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols <em>in vivo</em> and <em>in vitro</em>. Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases.</div></div>\",\"PeriodicalId\":342,\"journal\":{\"name\":\"iScience\",\"volume\":\"27 12\",\"pages\":\"Article 111334\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-11-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"iScience\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589004224025598\",\"RegionNum\":2,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"iScience","FirstCategoryId":"103","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589004224025598","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

溶酶体生物硫醇在许多细胞过程和疾病中发挥着关键作用。研究一种实时标记溶酶体中生物硫醇的有效方法意义重大、迫在眉睫，因为它可以为相关疾病的诊断提供重要信息。在这项研究中，我们开发了一种溶酶体靶向荧光探针（LY-DCM-P），它具有 150 nm 的大斯托克斯位移，可在体内和体外灵敏、选择性地检测生物硫醇。此外，LY-DCM-P 显示出较低的细胞毒性和出色的溶酶体靶向能力。该探针被成功用于监测各种生命系统中溶酶体生物硫醇的波动，为准确监测生物硫醇相关疾病的发生和进展提供了巨大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro

查看原文本刊更多论文

A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro

Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols in vivo and in vitro. Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

期刊介绍： Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.