用光学显微镜研究昆虫几丁质化组织的组织学程序。

IF 2.5 3区工程技术 Q1 MICROSCOPY

Micron Pub Date : 2024-11-13 DOI:10.1016/j.micron.2024.103744

Manuela Gallardo , María Valladolid

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引用次数: 0

摘要

我们介绍了一种获得昆虫角质层组织学制备物的方法，以便在光学显微镜下对其进行观察。使用 EDTA（乙二胺四乙酸）作为几丁质中某些金属的螯合剂，可以软化组织并使我们能够切割它们。通过两种不同的方法将组织嵌入塑料树脂中，然后分别用低温恒温器（20-30 μm）和超微切片机（4 μm）进行切割。这一程序以传统的组织学产品为基础，使我们能够获得可在光学显微镜下观察的制片，便于研究鳞片的内部结构，例如观察不同的层次或结构，如腺体或刚毛。

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A histological procedure for the study of insect chitinized tissues in light microscopy

We present a procedure to obtain histological preparations of insect cuticles for their observation in ligth microscopy. The use of EDTA (ethylendiaminetetraacetic acid), as a chelator of certain metals present in chitin, soften the tissues and allows us to cut them. The tissues were embbeding in plastic resines in two different protocols and cutted by cryostat (20–30 μm) and ultramicrotome (4 μm) respectively. This procedure, based on traditional histology products, allows us to obtain preparations observable under an optical microscope, facilitating the study of the internal structure of the cuticule, e.g. visualizing the different layers or structures such as glands or setae.

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来源期刊

Micron 工程技术-显微镜技术

CiteScore

4.30

自引率

4.20%

发文量

100

审稿时长

31 days

期刊介绍： Micron is an interdisciplinary forum for all work that involves new applications of microscopy or where advanced microscopy plays a central role. The journal will publish on the design, methods, application, practice or theory of microscopy and microanalysis, including reports on optical, electron-beam, X-ray microtomography, and scanning-probe systems. It also aims at the regular publication of review papers, short communications, as well as thematic issues on contemporary developments in microscopy and microanalysis. The journal embraces original research in which microscopy has contributed significantly to knowledge in biology, life science, nanoscience and nanotechnology, materials science and engineering.