利用 MitoPlates™ 和突触线粒体对突触线粒体进行代谢表型。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4264-1_4

Aleksandra Stawikowska, Magdalena Dziembowska, Bozena Kuzniewska

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引用次数: 0

摘要

通过使用 MitoPlates™ S-1 进行线粒体功能测试，我们可以了解线粒体底物代谢的特征。MitoPlates™ 是预涂有多种底物的 96 孔微孔板。线粒体底物产生的 NADH 和 FADH2 的电子流根据作为终端电子受体的氧化还原染料的还原情况进行测量。在这里，我们描述了如何应用 MitoPlates™ 来描述封闭在分离的突触前后末端（突触神经体）中的突触线粒体的新陈代谢。

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Metabolic Phenotyping of Synaptic Mitochondria Using MitoPlates™ and Synaptoneurosomes.

Mitochondrial functional assays using MitoPlates™ S-1 allow us to characterize mitochondria in terms of substrate metabolism. MitoPlates™ are 96-well microplates pre-coated with a diverse set of substrates. The electron flow from NADH and FADH₂ producing mitochondrial substrates is measured based on the reduction of redox dye, that acts as a terminal electron acceptor. Here, we describe the application of MitoPlates™ to characterize the metabolism of synaptic mitochondria enclosed in isolated pre- and postsynaptic terminals (synaptoneurosomes).

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.