使用手持式中空纤维法评估中空纤维孔径和膜材料对病毒浓度的影响。

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-11-14 DOI:10.1016/j.jviromet.2024.115065

Shinsuke Higuchi , Tatsuki Satou , Yuki Uchida

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引用次数: 0

摘要

使用中空纤维浓缩病毒被广泛用于疫苗生产，以在低剪切应力下保持病毒的感染性，并使生产规模更容易扩大。然而，在疫苗开发的早期阶段，研究实验室可用的病毒材料往往有限，因此很难找到最佳的中空纤维。此外，关于优化中空纤维孔径和膜结构以提高病毒浓度的研究文章也寥寥无几。本研究对之前建立的手持式中空纤维病毒浓缩方法进行了改进，以减少所需的样品量，并实现简单易行的中空纤维筛选。以寨卡病毒为模型，证实了手持式中空纤维病毒浓缩筛选方法的有效性。

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Evaluation of the impact of hollow fiber pore size and membrane material on virus concentration using a handheld hollow fiber method

Virus concentration using hollow fibers is widely used for vaccine production to maintain viral infectivity with low shear stress and to allow for easier scale-up of production. However, research laboratories often have limited available viral materials at the early stage of vaccine development, making it difficult to find an optimal hollow-fiber. In addition, few research articles have reported on optimizing hollow fiber pore size and membrane structure for virus concentration. In this study, the previously established handheld hollow fiber virus concentration method was modified to reduce the sample volume required and to enable simple and easy hollow fiber screening. The handheld hollow fiber screening method for virus concentration was confirmed to be effective using Zika as a model virus.

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.