Hanqing Lin, Yu Heng, Xiaoke Zhu, Tian Wang, Lei Tao
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CD3<sup>+</sup> T lymphocyte counts decreased significantly, and the CD4<sup>+</sup>/CD8<sup>+</sup> ratio increased in HPSCC patients following radiation. The secretion of IFN-γ from peripheral CD8+ T lymphocytes was significantly reduced after irradiation, while the secretion of TNF-α and perforin did not change significantly. Furthermore, the proliferation capacity of peripheral CD8<sup>+</sup> T lymphocytes was decreased following RT.</p><p><strong>Conclusions: </strong>RT significantly decreased the number of peripheral T lymphocytes and impaired the secretory function and proliferation ability of CD8<sup>+</sup> T lymphocytes in HPSCC patients. These findings provide insight into the mechanisms underlying the therapeutic effects of RT on HPSCC and have implications for optimizing treatment strategies.</p>","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of radiotherapy on peripheral T lymphocytes in hypopharyngeal squamous cell carcinoma.\",\"authors\":\"Hanqing Lin, Yu Heng, Xiaoke Zhu, Tian Wang, Lei Tao\",\"doi\":\"10.1016/j.oooo.2024.10.080\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>To investigate the impact of radiotherapy (RT) on the distribution and function of peripheral CD8<sup>+</sup> T lymphocytes in patients with hypopharyngeal squamous cell carcinoma (HPSCC).</p><p><strong>Methods: </strong>A total of 105 HPSCC patients who underwent definitive RT were enrolled. Baseline levels of peripheral immune cells were obtained, and their alteration during RT was evaluated. Flow cytometry was used to analyze T-cell distribution, cytokine secretion, and CD8<sup>+</sup> T lymphocyte proliferation capacity.</p><p><strong>Results: </strong>Lymphocyte count significantly decreased following radiation and remained in a low level after 1 year of RT. CD3<sup>+</sup> T lymphocyte counts decreased significantly, and the CD4<sup>+</sup>/CD8<sup>+</sup> ratio increased in HPSCC patients following radiation. The secretion of IFN-γ from peripheral CD8+ T lymphocytes was significantly reduced after irradiation, while the secretion of TNF-α and perforin did not change significantly. 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引用次数: 0
摘要
背景：研究放疗（RT）对下咽鳞状细胞癌（HPSCC）患者外周 CD8+ T 淋巴细胞分布和功能的影响：研究放疗（RT）对下咽鳞状细胞癌（HPSCC）患者外周CD8+ T淋巴细胞分布和功能的影响：方法：共招募了105名接受明确RT治疗的下咽鳞状细胞癌患者。获得了外周免疫细胞的基线水平，并评估了它们在 RT 期间的变化。流式细胞术用于分析 T 细胞分布、细胞因子分泌和 CD8+ T 淋巴细胞增殖能力：结果：淋巴细胞计数在放射后明显下降，RT 1 年后仍处于较低水平。放疗后，HPSCC 患者的 CD3+ T 淋巴细胞数量明显减少，CD4+/CD8+ 比率增加。照射后，外周 CD8+ T 淋巴细胞分泌的 IFN-γ 明显减少，而 TNF-α 和穿孔素的分泌则无明显变化。此外，外周 CD8+ T 淋巴细胞的增殖能力在 RT 后也有所下降：结论：RT明显降低了HPSCC患者外周T淋巴细胞的数量，损害了CD8+ T淋巴细胞的分泌功能和增殖能力。这些发现深入揭示了RT对HPSCC治疗作用的机制，对优化治疗策略具有重要意义。
Impact of radiotherapy on peripheral T lymphocytes in hypopharyngeal squamous cell carcinoma.
Background: To investigate the impact of radiotherapy (RT) on the distribution and function of peripheral CD8+ T lymphocytes in patients with hypopharyngeal squamous cell carcinoma (HPSCC).
Methods: A total of 105 HPSCC patients who underwent definitive RT were enrolled. Baseline levels of peripheral immune cells were obtained, and their alteration during RT was evaluated. Flow cytometry was used to analyze T-cell distribution, cytokine secretion, and CD8+ T lymphocyte proliferation capacity.
Results: Lymphocyte count significantly decreased following radiation and remained in a low level after 1 year of RT. CD3+ T lymphocyte counts decreased significantly, and the CD4+/CD8+ ratio increased in HPSCC patients following radiation. The secretion of IFN-γ from peripheral CD8+ T lymphocytes was significantly reduced after irradiation, while the secretion of TNF-α and perforin did not change significantly. Furthermore, the proliferation capacity of peripheral CD8+ T lymphocytes was decreased following RT.
Conclusions: RT significantly decreased the number of peripheral T lymphocytes and impaired the secretory function and proliferation ability of CD8+ T lymphocytes in HPSCC patients. These findings provide insight into the mechanisms underlying the therapeutic effects of RT on HPSCC and have implications for optimizing treatment strategies.
期刊介绍：
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.
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