福尔马林固定石蜡包埋组织代谢组学和蛋白质组学统一分析规程。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-11-09 DOI:10.1016/j.xpro.2024.103442

Abisola R Isaiah, Laneke Luies, Du Toit Loots, Aurelia A Williams, Maré Vlok, Novel N Chegou, Marceline Tutu van Furth, Martijn van der Kuip, Shayne Mason

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引用次数: 0

摘要

使用档案福尔马林固定石蜡包埋（FFPE）组织样本进行生化分析存在问题，因为会形成希夫碱，导致分析提取过程中蛋白质和代谢物的产量较低。在此，我们采用统一的方案对 FFPE 组织进行代谢组学和蛋白质组学分析，从而克服了这一问题。使用 20 毫克湿质量组织，该方案可持续提取 50 多种代谢物（涉及 11 类代谢物）和 900 多种蛋白质。

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Protocol for unified metabolomics and proteomics analysis of formalin-fixed paraffin-embedded tissue.

The use of archival formalin-fixed paraffin-embedded (FFPE) tissue samples for biochemical analyses is problematic because of the formation of a Schiff base, leading to low protein and metabolite yields during analytical extractions. Here, we overcome this issue using a unified protocol on FFPE tissue for metabolomics and proteomics analyses. Using 20 mg of wet mass tissue, this protocol consistently extracted more than 50 metabolites (across 11 classes of metabolites) and over 900 proteins.

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