气道上皮细胞通过激活 Wnt/β-catenin 通路促进体外气道平滑肌细胞增殖。

IF 1.9 4区 医学 Q3 PHYSIOLOGY
Yilun Liu , Jiana Li , Rongchang Chen , Fei Shi , Yi Xiong
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引用次数: 0

摘要

哮喘是一种常见的慢性气道炎症性疾病,给社会和个人带来巨大的健康和经济负担。目前的治疗方法主要集中在缓解症状和改善肺功能上,往往不能从根本上解决病理问题。因此,探索新的治疗方法至关重要。气道平滑肌细胞(ASMC)在调节气道张力和气流方面起着关键作用,而气道平滑肌细胞的异常增殖会导致哮喘患者的气道重塑。气道上皮细胞(AECs)是抵御病原体和过敏原的第一道屏障,也具有重要的免疫功能。本研究的重点是气道上皮细胞和 ASMC 之间的相互作用,因为气道上皮细胞位于气道表面,更容易给药。研究这种关系可促进针对 AECs 的新型干预措施,从而抑制病理 ASMCs 的活性。在我们的实验中,我们从健康小鼠体内分离出了 ASMC 和 AECs,并发现 AECs 在共培养过程中能显著促进 ASMCs 的增殖。RNA 测序显示,这一过程可能与 ASMCs 中典型 Wnt 信号通路的激活有关。通过使用 Wnt 通路抑制剂(endo-IWR1)和 siRNA 破坏 Wnt 受体,我们逆转了这种表型。这一发现表明,AECs 可通过激活 ASMC 中的 Wnt 通路来促进 ASMC 的增殖。Wnt/β-catenin通路似乎在ASMCs增殖中发挥了重要作用,这表明未来的病理研究应考虑Wnt通路在气道重塑中的潜在参与。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Airway epithelial cells promote in vitro airway smooth muscle cell proliferation by activating the Wnt/β-catenin pathway
Asthma is a common chronic inflammatory airway disease, imposing a substantial health and economic burden on society and individuals. Current treatments primarily focus on symptom relief and lung function improvement, often failing to address the underlying pathology. Thus, exploring new therapeutic approaches is crucial. Airway smooth muscle cells (ASMCs) play a key role in regulating airway tone and airflow, while abnormal ASMCs proliferation contributes to airway remodeling in asthma. Airway epithelial cells (AECs), serving as the first barrier against pathogens and allergens, also have critical immune functions.
This study focuses on the interaction between AECs and ASMCs, as AECs are more accessible for drug delivery due to their location at the airway surface. Investigating this relationship could facilitate novel interventions targeting AECs to inhibit pathological ASMCs activity. In our experiment, we isolated ASMCs and AECs from healthy mice and found that AECs significantly promoted ASMCs proliferation in co-culture. RNA sequencing revealed that this process might be linked to the activation of the canonical Wnt signaling pathway in ASMCs. By using Wnt pathway inhibitors (endo-IWR1) and siRNA to disrupt Wnt receptors, we reversed this phenotype. This finding suggests that AECs may promote ASMCs proliferation by activating the Wnt pathway in ASMCs. The Wnt/β-catenin pathway appears to play an important role in ASMCs proliferation, indicating that future pathological studies should consider the potential involvement of the Wnt pathway in airway remodeling.
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来源期刊
CiteScore
4.80
自引率
8.70%
发文量
104
审稿时长
54 days
期刊介绍: Respiratory Physiology & Neurobiology (RESPNB) publishes original articles and invited reviews concerning physiology and pathophysiology of respiration in its broadest sense. Although a special focus is on topics in neurobiology, high quality papers in respiratory molecular and cellular biology are also welcome, as are high-quality papers in traditional areas, such as: -Mechanics of breathing- Gas exchange and acid-base balance- Respiration at rest and exercise- Respiration in unusual conditions, like high or low pressure or changes of temperature, low ambient oxygen- Embryonic and adult respiration- Comparative respiratory physiology. Papers on clinical aspects, original methods, as well as theoretical papers are also considered as long as they foster the understanding of respiratory physiology and pathophysiology.
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