从结直肠癌切除样本中建立患者衍生有机体

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-11-10 DOI:10.1007/7651_2024_575

Didem Seven, Szilvia Baron, Henner F Farin

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引用次数: 0

摘要

结直肠癌（CRC）器官组织可作为强大的临床前细胞模型，准确反映个体肿瘤特征。建立源自患者的 CRC 生物库有助于广泛的应用，包括肿瘤学基础研究、新药发现、药物测试和个性化医疗。本章详细介绍了从原发性和转移性 CRC 手术样本以及肿瘤邻近的正常结肠组织中生成器官组织的过程。此外，还介绍了培养和冷冻保存 CRC 有机体的最佳实践。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Establishment of Patient-Derived Organoids from Colorectal Cancer Resection Samples.

Colorectal cancer (CRC) organoids can serve as powerful preclinical cell models that accurately reflect individual tumor characteristics. Establishing a patient-derived CRC biobank facilitates a wide range of applications, including basic oncology research, new drug discovery, drug testing, and personalized medicine. This chapter details the process of generation of organoids from surgical samples of primary and metastatic CRC as well as from tumor adjacent normal colon tissues. Furthermore, best practices for cultivation and cryostorage of CRC organoids are described.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.