母马初乳免疫球蛋白对马驹体内 TLR3、TLR4 和 TLR7 表达的影响。

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Anna Migdał, Łukasz Migdał, Maria Oczkowicz, Barbara Tombarkiewicz, Adam Okólski
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引用次数: 0

摘要

初乳含有激素、酶、多酰胺、核酸衍生物和氨基酸衍生物等物质,还包括免疫调节物质、富含脯氨酸的多肽、免疫球蛋白(Ig)以及抑菌化合物。研究的目的是评估初乳 Ig 对某些 Toll 样受体基因(TLR3、TLR4 和 TLR7)表达水平的影响。实验对象是 25 匹波兰矮种马。按照以下方案采集血液样本:初乳前、1、3、5、10、20 和 30 日龄时。初乳在第一次哺乳前、分娩后 24 小时和 3 天采集。使用商业试剂盒 TaqMan®MGB 探针在 Illumina Eco 上进行基因表达分析。初乳质量通过折射仪和 BRIX 值进行评估。总 Ig 水平通过分光光度法计算。IgG水平通过ELISA检测法进行测量。初乳的质量与 TLR4 和 TLR7 的表达明显相关。摄入 Ig 含量低的初乳的小马驹的 TLR4 基因表达水平较高,而 TLR7 的表达水平则呈相反趋势。这一结果表明,初乳可能在形成免疫系统对细菌病原体的反应机制方面发挥着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of the mare colostral immunoglobulins on the expression of TLR3, TLR4, and TLR7 in foals

Colostrum contains substances such as hormones, enzymes, polyamides, nucleic acid derivatives, and amino acid derivatives and also includes immunomodulating substances, proline-rich polypeptides, immunoglobulins (Ig) as well as bacteriostatic compounds. The aim of the study was to evaluate the impact of colostral Ig on the level of expression of selected Toll-like receptor genes (TLR3, TLR4, and TLR7). The experiments were conducted on 25 Polish Pony foals. Blood samples were collected according to the following scheme: before first suckling, at the 1st, 3rd, 5th, 10th, 20th, and 30th, day of age. Colostrum was obtained before the first suckling, 24 hr, and 3 days after the delivery. Gene expression analyses were performed on Illumina Eco using a commercial kit TaqMan®MGB probes. The quality of colostrum was assessed via refractometer and BRIX value. The total Ig level was calculated from the spectrophotometric method. IgG levels were measured using ELISA assay. The quality of colostrum significantly correlated with TLR4 and TLR7 expression. Foals that ingested colostrum with low Ig content displayed a higher level of TLR4 gene expression, while in the case of TLR7 the opposite trend was shown. This result indicates that colostrum may play an important role in shaping the mechanisms of building the immune system's response to bacterial pathogens.

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来源期刊
Animal Science Journal
Animal Science Journal 生物-奶制品与动物科学
CiteScore
3.80
自引率
5.00%
发文量
111
审稿时长
1 months
期刊介绍: Animal Science Journal (a continuation of Animal Science and Technology) is the official journal of the Japanese Society of Animal Science (JSAS) and publishes Original Research Articles (full papers and rapid communications) in English in all fields of animal and poultry science: genetics and breeding, genetic engineering, reproduction, embryo manipulation, nutrition, feeds and feeding, physiology, anatomy, environment and behavior, animal products (milk, meat, eggs and their by-products) and their processing, and livestock economics. Animal Science Journal will invite Review Articles in consultations with Editors. Submission to the Journal is open to those who are interested in animal science.
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