Chengli Lou, Yijing Zhou, Jian Liao, Xiuqin Xu, Ke Huang, Xiaoping Fan, Pingxin Hu, Xiangjing Wang, Bo Feng, Fuxiang Zhu
{"title":"清热解毒方对特发性膜性肾病小鼠Th17细胞和Tregs的影响","authors":"Chengli Lou, Yijing Zhou, Jian Liao, Xiuqin Xu, Ke Huang, Xiaoping Fan, Pingxin Hu, Xiangjing Wang, Bo Feng, Fuxiang Zhu","doi":"10.62347/HJVG8103","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to evaluate the therapeutic effect of the QingreHuoxue formula on mice with Idiopathic Membranous Nephropathy (IMN) and its impact on Th17 cells and Tregs.</p><p><strong>Methods: </strong>A mouse model of IMN was established, and the mice were treated with traditional Chinese medicine, western medicine, or a combination of both. The efficacy and immunomodulatory effects of the QingreHuoxue formula were evaluated by examining renal pathology, urinary protein levels, peripheral blood Th17 and Treg cell counts, and comparing the expression levels of IL-17 and transforming growth factor-β1 in renal tissues.</p><p><strong>Results: </strong>Compared to the untreated IMN model group, the IMN mice treated with TCM, western medicine, or the combination showed significant improvements in proteinuria, renal pathology, peripheral T lymphocyte counts, and IL-17 expression in renal tissues. Notably, the group treated with a combination of Chinese and western medicine exhibited better outcomes than the group treated with western medicine alone.</p><p><strong>Conclusions: </strong>The QingreHuoxue formula was effective in reducing proteinuria, modulating T cell immune function, and protecting renal tissue in mice with IMN.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"16 10","pages":"5326-5336"},"PeriodicalIF":1.7000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11558394/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of QingreHuoxue formula on Th17 cells and Tregs in mice with idiopathic membranous nephropathy.\",\"authors\":\"Chengli Lou, Yijing Zhou, Jian Liao, Xiuqin Xu, Ke Huang, Xiaoping Fan, Pingxin Hu, Xiangjing Wang, Bo Feng, Fuxiang Zhu\",\"doi\":\"10.62347/HJVG8103\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>This study aimed to evaluate the therapeutic effect of the QingreHuoxue formula on mice with Idiopathic Membranous Nephropathy (IMN) and its impact on Th17 cells and Tregs.</p><p><strong>Methods: </strong>A mouse model of IMN was established, and the mice were treated with traditional Chinese medicine, western medicine, or a combination of both. The efficacy and immunomodulatory effects of the QingreHuoxue formula were evaluated by examining renal pathology, urinary protein levels, peripheral blood Th17 and Treg cell counts, and comparing the expression levels of IL-17 and transforming growth factor-β1 in renal tissues.</p><p><strong>Results: </strong>Compared to the untreated IMN model group, the IMN mice treated with TCM, western medicine, or the combination showed significant improvements in proteinuria, renal pathology, peripheral T lymphocyte counts, and IL-17 expression in renal tissues. Notably, the group treated with a combination of Chinese and western medicine exhibited better outcomes than the group treated with western medicine alone.</p><p><strong>Conclusions: </strong>The QingreHuoxue formula was effective in reducing proteinuria, modulating T cell immune function, and protecting renal tissue in mice with IMN.</p>\",\"PeriodicalId\":7731,\"journal\":{\"name\":\"American journal of translational research\",\"volume\":\"16 10\",\"pages\":\"5326-5336\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11558394/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American journal of translational research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.62347/HJVG8103\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of translational research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.62347/HJVG8103","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0
摘要
研究目的本研究旨在评估清热解毒方对特发性膜性肾病（IMN）小鼠的治疗效果及其对Th17细胞和Tregs的影响：方法：建立特发性膜性肾病小鼠模型，用中药、西药或两者联合治疗小鼠。通过检查肾脏病理、尿蛋白水平、外周血Th17和Treg细胞计数，并比较肾组织中IL-17和转化生长因子-β1的表达水平，评价清热解毒方的疗效和免疫调节作用：结果：与未经治疗的IMN模型组相比，接受中药、西药或联合治疗的IMN小鼠在蛋白尿、肾脏病理变化、外周T淋巴细胞计数和肾组织中IL-17表达方面均有显著改善。值得注意的是，中西医结合治疗组的疗效优于单纯西医治疗组：结论：清热解毒方能有效减少蛋白尿、调节 T 细胞免疫功能并保护 IMN 小鼠的肾组织。
Effect of QingreHuoxue formula on Th17 cells and Tregs in mice with idiopathic membranous nephropathy.
Objectives: This study aimed to evaluate the therapeutic effect of the QingreHuoxue formula on mice with Idiopathic Membranous Nephropathy (IMN) and its impact on Th17 cells and Tregs.
Methods: A mouse model of IMN was established, and the mice were treated with traditional Chinese medicine, western medicine, or a combination of both. The efficacy and immunomodulatory effects of the QingreHuoxue formula were evaluated by examining renal pathology, urinary protein levels, peripheral blood Th17 and Treg cell counts, and comparing the expression levels of IL-17 and transforming growth factor-β1 in renal tissues.
Results: Compared to the untreated IMN model group, the IMN mice treated with TCM, western medicine, or the combination showed significant improvements in proteinuria, renal pathology, peripheral T lymphocyte counts, and IL-17 expression in renal tissues. Notably, the group treated with a combination of Chinese and western medicine exhibited better outcomes than the group treated with western medicine alone.
Conclusions: The QingreHuoxue formula was effective in reducing proteinuria, modulating T cell immune function, and protecting renal tissue in mice with IMN.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
