N Z Mirzoeva, S O Pipiya, Yu A Mokrushina, M V Serebryakova, A A Grigoreva, S A Dubiley, S S Terekhov, I V Smirnov
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引用次数: 0
摘要
由于微生物对抗生素的耐药性不断增加,我们必须寻找新的抗菌剂。由于肽具有基因编码特性,因此有望成为新型抗菌药物的候选物质。兰肽 andalusicin 对革兰氏阳性菌具有显著的抗菌活性,因此是开发 DNA 编码兰生素文库的理想支架。本研究在异源大肠杆菌系统中重建了第三类鹅掌楸碱合成酶 AncKC 对鹅掌楸碱的修饰反应。研究结果为创造基于多肽的新型抗菌剂提供了可能性。
Reconstruction of the Reaction of Andalusicin Lantibiotic Modification by Lanthionine Synthetase AncKC in a Heterologous Escherichia coli System.
The increasing resistance of microorganisms to antibiotics makes it a necessity that we search for new antimicrobial agents. Due to their genetically encoded nature, peptides are promising candidates for new antimicrobial drugs. Lantipeptide andalusicin exhibits significant antimicrobial activity against Gram-positive bacteria, making it a promising scaffold for the development of DNA-encoded libraries of lantibiotics. In this study, the modification reaction of andalusicin by class III lanthionine synthetase AncKC was reconstructed in a heterologous Escherichia coli system. The results obtained open possibilities for creating novel peptide- based antimicrobial agents.
期刊介绍:
Acta Naturae is an international journal on life sciences based in Moscow, Russia.
Our goal is to present scientific work and discovery in molecular biology, biochemistry, biomedical disciplines and biotechnology. These fields represent the most important priorities for the research and engineering development both in Russia and worldwide. Acta Naturae is also a periodical for those who are curious in various aspects of biotechnological business, innovations in pharmaceutical areas, intellectual property protection and social consequences of scientific progress. The journal publishes analytical industrial surveys focused on the development of different spheres of modern life science and technology.
Being a radically new and totally unique journal in Russia, Acta Naturae is useful to both representatives of fundamental research and experts in applied sciences.