云皮解毒化瘤汤通过 Wnt/β-catenin 信号通路减轻哮喘小鼠的气道炎症和气道重塑。

IF 2.5 4区 医学 Q3 ALLERGY
Allergologia et immunopathologia Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI:10.15586/aei.v52i6.1210
Li Du, Zhu Chen, Qiong Tao, Jianhui Yang, Na Chen, Qiao Wang
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引用次数: 0

摘要

背景:哮喘是儿童中最常见的慢性呼吸道疾病之一,严重损害了患者的健康,并给医疗系统带来了日益沉重的负担。通过对动物模型的研究,几种传统中药在缓解哮喘症状方面具有显著疗效。最近的研究表明,云皮解毒化喘汤在治疗痰阻型肺哮喘方面具有显著疗效。然而,云皮解毒化喘汤对哮喘进展的确切调节作用还需要进一步研究:材料与方法:材料:成功建立了由卵清蛋白(OVA)诱发的哮喘大鼠模型。材料和方法:成功建立了由卵清蛋白(OVA)诱发的哮喘大鼠模型,并通过苏木精和伊红(H&E)染色检测了肺组织的病理变化。通过酶联免疫吸附血清分析法(ELISA)检测白细胞介素6(IL-6)和IL-1β的水平。支气管肺泡灌洗液中的总细胞数或嗜酸性粒细胞数由细胞计数器确认。通过马森染色法评估支气管中的胶原沉积。结果:结果:这项研究表明,YPD 可以减轻 OVA 引发的哮喘大鼠模型的气道炎症。此外,研究还发现 YPD 可减少肺部炎症细胞因子的产生,并抑制支气管肺泡灌洗液中炎症细胞的浸润。此外,经 YPD 处理后,OVA 刺激的气道重塑也会受到抑制。最后,研究还发现,在 OVA 刺激的哮喘大鼠模型中,YPD 可抑制无翼鸟相关整合位点-β-catenin(Wnt/β-catenin)信号通路:结论:YPD可通过Wnt/β-catenin信号通路缓解哮喘小鼠的气道炎症和重塑。这项研究为 YPD 在哮喘治疗中的潜在应用提供了重要启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Yunpi Xiefei Huatan Tang decoction reduces airway inflammation and airway remodeling in asthmatic mice through Wnt/β-catenin signaling pathway.

Background: Asthma is one of the most prevalent chronic respiratory diseases among children, markedly impairing patient's health and imposing an increasing burden on the healthcare system. Several traditional Chinese medicines have demonstrated efficacy in alleviating asthma symptoms through studies conducted on animal models. Recent studies have shown that the Yunpi Xiefei Huatan Tang decoction (YPD) exhibits significant therapeutic outcomes in treating phlegm-obstructed pulmonary asthma. However, the precise regulatory effects of YPD on the progression of asthma require additional investigation.

Objective: To explore the functions of YPD in asthma progression.

Material and methods: The asthma rat model triggered by ovalbumin (OVA) was established successfully. The pathological changes of lung tissues were examined through Hematoxylin and Eosin (H&E) staining. The levels of Interleukin 6 (IL-6) and IL-1β were tested through Enzyme-Linked-Immunosorbent Serologic Assay (ELISA). The number of total cells or eosinophils in bronchoalveolar lavage fluid was confirmed through cell counter. The collagen deposition in bronchi was assessed through Masson staining. The protein expressions were measured through western blot.

Results: This study demonstrated that YPD could mitigate airway inflammation in an OVA-triggered asthma rat model. Furthermore, YPD was found to decrease the production of inflammatory cytokines in the lungs and suppress the infiltration of inflammatory cells into bronchoalveolar lavage fluid. Additionally, the airway remodeling stimulated by OVA could be suppressed following YPD treatment. Finally, it was disclosed that YPD inhibited the wingless-related integration site-beta-catenin (Wnt/β-catenin) signaling pathway in the OVA-stimulated asthma rat model.

Conclusion: YPD alleviated airway inflammation and remodeling in asthmatic mice via the Wnt/β-catenin signaling pathway. This research offers significant insights into the potential application of YPD in the treatment of asthma.

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来源期刊
CiteScore
3.70
自引率
0.00%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.
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