利用生物炭辅助零价铁自养生物还原法去除地下水中的铬（VI）和硝酸盐：提高电子转移效率和减少 EPS 积累

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2024-11-14 DOI:10.1016/j.envpol.2024.125313

Chuanjin Xia, Weidong Cheng, Meng Ren, Yuling Zhu

{"title":"利用生物炭辅助零价铁自养生物还原法去除地下水中的铬（VI）和硝酸盐：提高电子转移效率和减少 EPS 积累","authors":"Chuanjin Xia, Weidong Cheng, Meng Ren, Yuling Zhu","doi":"10.1016/j.envpol.2024.125313","DOIUrl":null,"url":null,"abstract":"Current strategies primarily utilize heterotrophic or mixotrophic bioreduction for the simultaneous removal of Cr(VI) and NO3- from groundwater. However, given the oligotrophic nature of groundwater, autotrophic bioreduction could be more appropriate, though it remains notably underdeveloped. Here, an autotrophic bioreduction technology utilizing biochar (BC)-assisted zero valent iron (ZVI) is proposed. The pyrolysis temperature of BC was optimized to enhance electron transfer efficiency and reduce extracellular polymeric substances (EPS) accumulation. BC500, with the superior electron transfer capabilities, was the most effective. After an 11-week period, the ZVI+BC500 biotic column still achieved 100% removal efficiency for Cr(VI) and 93.37±0.33% for NO3-, with initial concentrations of 26 mg/L and 50 mg/L, respectively. Its performance significantly surpasses that of ZVI alone, effectively reducing the interference of Cr(VI) on denitrification. The presence of quinone and phenolic compounds in BC500, serving as electron-accepting and electron-donating groups, improves the efficiency of electron transfer between ZVI and microbes. Metagenomic analysis showed an increase in the growth of autotrophic bacteria such as Hydrogenophaga spp. and Rhodanobacter denitrificans, and heterotrophic bacteria including Arenimonas daejeonensis and Chryseobacterium shandongense. The promotion facilitates the expression of genes associated with Cr(VI) reduction (chrR, nemA) and denitrification (narG, nirS). BC500 also enhanced EPS production, which facilitates the adsorption and reduction of Cr(VI), mitigating its inhibitory effects on denitrification. Notably, in the ZVI+BC500 biotic column, the accumulated EPS primarily consists of loosely bound EPS rather than tightly bound EPS, potentially reducing the risk of pore clogging during in-situ groundwater treatment.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"15 1","pages":""},"PeriodicalIF":7.6000,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chromium(VI) and Nitrate Removal from Groundwater Using Biochar-Assisted Zero Valent Iron Autotrophic Bioreduction: Enhancing Electron Transfer Efficiency and Reducing EPS Accumulation\",\"authors\":\"Chuanjin Xia, Weidong Cheng, Meng Ren, Yuling Zhu\",\"doi\":\"10.1016/j.envpol.2024.125313\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Current strategies primarily utilize heterotrophic or mixotrophic bioreduction for the simultaneous removal of Cr(VI) and NO3- from groundwater. However, given the oligotrophic nature of groundwater, autotrophic bioreduction could be more appropriate, though it remains notably underdeveloped. Here, an autotrophic bioreduction technology utilizing biochar (BC)-assisted zero valent iron (ZVI) is proposed. The pyrolysis temperature of BC was optimized to enhance electron transfer efficiency and reduce extracellular polymeric substances (EPS) accumulation. BC500, with the superior electron transfer capabilities, was the most effective. After an 11-week period, the ZVI+BC500 biotic column still achieved 100% removal efficiency for Cr(VI) and 93.37±0.33% for NO3-, with initial concentrations of 26 mg/L and 50 mg/L, respectively. Its performance significantly surpasses that of ZVI alone, effectively reducing the interference of Cr(VI) on denitrification. The presence of quinone and phenolic compounds in BC500, serving as electron-accepting and electron-donating groups, improves the efficiency of electron transfer between ZVI and microbes. Metagenomic analysis showed an increase in the growth of autotrophic bacteria such as Hydrogenophaga spp. and Rhodanobacter denitrificans, and heterotrophic bacteria including Arenimonas daejeonensis and Chryseobacterium shandongense. The promotion facilitates the expression of genes associated with Cr(VI) reduction (chrR, nemA) and denitrification (narG, nirS). BC500 also enhanced EPS production, which facilitates the adsorption and reduction of Cr(VI), mitigating its inhibitory effects on denitrification. Notably, in the ZVI+BC500 biotic column, the accumulated EPS primarily consists of loosely bound EPS rather than tightly bound EPS, potentially reducing the risk of pore clogging during in-situ groundwater treatment.\",\"PeriodicalId\":311,\"journal\":{\"name\":\"Environmental Pollution\",\"volume\":\"15 1\",\"pages\":\"\"},\"PeriodicalIF\":7.6000,\"publicationDate\":\"2024-11-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Pollution\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.envpol.2024.125313\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollution","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.envpol.2024.125313","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

目前的策略主要是利用异养或混养生物还原法同时去除地下水中的六价铬和三氧化二氮。然而，考虑到地下水的低营养性，自养生物还原可能更为合适，但其发展仍明显不足。本文提出了一种利用生物炭（BC）辅助零价铁（ZVI）的自养生物还原技术。对生物炭的热解温度进行了优化，以提高电子传递效率并减少胞外聚合物物质（EPS）的积累。电子传递能力更强的 BC500 最为有效。经过 11 周的试验，ZVI+BC500 生物柱对六价铬的去除率仍然达到 100%，对 NO3- 的去除率为 93.37±0.33%（初始浓度分别为 26 mg/L 和 50 mg/L）。其性能大大超过了单独使用 ZVI 的效果，有效降低了 Cr(VI) 对反硝化的干扰。BC500 中的醌类和酚类化合物可作为电子受体和电子供体，提高了 ZVI 与微生物之间的电子传递效率。元基因组分析表明，Hydrogenophaga spp.和Rhodanobacter denitrificans等自养细菌以及Arenimonas daejeonensis和Chryseobacterium shandongense等异养细菌的生长都有所增加。这种促进作用有利于与六价铬还原（chrR、nemA）和反硝化（narG、nirS）相关的基因的表达。BC500 还提高了 EPS 的产量，这有利于吸附和还原六价铬，减轻其对反硝化的抑制作用。值得注意的是，在 ZVI+BC500 生物柱中，累积的 EPS 主要由松散结合的 EPS 组成，而不是紧密结合的 EPS，这可能会降低原位处理地下水过程中孔隙堵塞的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Chromium(VI) and Nitrate Removal from Groundwater Using Biochar-Assisted Zero Valent Iron Autotrophic Bioreduction: Enhancing Electron Transfer Efficiency and Reducing EPS Accumulation

查看原文本刊更多论文

Chromium(VI) and Nitrate Removal from Groundwater Using Biochar-Assisted Zero Valent Iron Autotrophic Bioreduction: Enhancing Electron Transfer Efficiency and Reducing EPS Accumulation

Current strategies primarily utilize heterotrophic or mixotrophic bioreduction for the simultaneous removal of Cr(VI) and NO₃^- from groundwater. However, given the oligotrophic nature of groundwater, autotrophic bioreduction could be more appropriate, though it remains notably underdeveloped. Here, an autotrophic bioreduction technology utilizing biochar (BC)-assisted zero valent iron (ZVI) is proposed. The pyrolysis temperature of BC was optimized to enhance electron transfer efficiency and reduce extracellular polymeric substances (EPS) accumulation. BC500, with the superior electron transfer capabilities, was the most effective. After an 11-week period, the ZVI+BC500 biotic column still achieved 100% removal efficiency for Cr(VI) and 93.37±0.33% for NO₃^-, with initial concentrations of 26 mg/L and 50 mg/L, respectively. Its performance significantly surpasses that of ZVI alone, effectively reducing the interference of Cr(VI) on denitrification. The presence of quinone and phenolic compounds in BC500, serving as electron-accepting and electron-donating groups, improves the efficiency of electron transfer between ZVI and microbes. Metagenomic analysis showed an increase in the growth of autotrophic bacteria such as Hydrogenophaga spp. and Rhodanobacter denitrificans, and heterotrophic bacteria including Arenimonas daejeonensis and Chryseobacterium shandongense. The promotion facilitates the expression of genes associated with Cr(VI) reduction (chrR, nemA) and denitrification (narG, nirS). BC500 also enhanced EPS production, which facilitates the adsorption and reduction of Cr(VI), mitigating its inhibitory effects on denitrification. Notably, in the ZVI+BC500 biotic column, the accumulated EPS primarily consists of loosely bound EPS rather than tightly bound EPS, potentially reducing the risk of pore clogging during in-situ groundwater treatment.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.