基于联吡啶钌-MOF 和双酶裂解技术的 "开-关-开 "双模式传感器对 K-ras 基因的灵敏检测。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2024-11-05 DOI:10.1016/j.bioelechem.2024.108845

Haotian Xie , Zhaojiang Yin , Guobin Wei , Binghui Li , Hanfeng Cui , Hao Fan , Jing Zhang

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引用次数: 0

摘要

该研究开发了一种基于双吡啶钌金属有机框架（Ru-MOF）和双酶裂解技术的双模式 "开-关-开 "传感器，用于灵敏检测 K-ras 基因。该传感器结合了电致化学发光（ECL）和荧光（FL）检测模式，通过催化发夹组装（CHA）和双酶裂解反应实现了对 K-ras 基因的高灵敏度和特异性检测。实验结果表明，该传感器对 K-ras 基因的检测限分别为 0.044 fM（ECL）和 0.16 fM（FL），具有极佳的选择性和检测稳定性。通过测试实际样品，该传感器显示出在复杂生物环境中的应用潜力。这种方法为癌症诊断和治疗提供了一种高效可靠的新工具。

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Sensitive detection of K-ras gene by a dual-mode “on-off-on” sensor based on bipyridine ruthenium-MOF and bis-enzymatic cleavage technology

This study developed a dual-mode “on-off-on” sensor based on a bipyridine ruthenium metal–organic framework (Ru-MOF) and dual enzyme cleavage technology for the sensitive detection of the K-ras gene. The sensor combines electrogenerated chemiluminescence (ECL) and fluorescence (FL) detection modes, achieving high sensitivity and specificity in detecting the K-ras gene through catalytic hairpin assembly (CHA) and dual enzyme cleavage reactions. Experimental results showed that the detection limits for the K-ras gene were 0.044 fM (ECL) and 0.16 fM (FL), demonstrating excellent selectivity and stability during detection. Through testing actual samples, the sensor has shown potential for application in complex biological environments. This method offers an efficient and reliable new tool for cancer diagnosis and treatment.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.