对纳米纤维素基复合材料和自体血凝块的骨诱导能力进行组织形态学比较评估。

IF 2 Q2 ORTHOPEDICS

Journal of Experimental Orthopaedics Pub Date : 2024-11-05 DOI:10.1002/jeo2.70067

Berik Tuleubayev, Yevgeniy Kamyshanskiy, Dina Saginova, Saule Akhmetova, Vladimir Vinokurov, Amina Koshanova, Yekaterina Kossilova

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引用次数: 0

摘要

目的：本研究旨在通过组织学和组织形态计量学评估，评估和比较以纳米纤维素（NFC）为基础的二水磷酸二钙和自体血凝块（ABC）复合材料对体内新骨组织形成的影响：共使用了 72 只股骨缺损（2 毫米）的大鼠。大鼠分为三组：(1) 用 ABC 填充缺损；(2) NFC-1--填充缺损区域的皮质板和髓内间隙；(3) NFC-2--仅填充缺损区域的髓内间隙。14天、30天和60天后，对骨缺损的愈合情况进行组织学和组织形态学分析：结果：NFC-2 组在第 30 天发现皮质板缺损完全闭合（p ＜结论：NFC-2 组的骨缺损完全闭合：该研究表明，位于皮质板水平以下、仅夯实髓内间隙（NFC-2）的基于 NFC 的复合材料可改善皮质板骨缺损部位的骨组织修复，并具有延长骨诱导的潜力：证据等级：不适用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Comparative histomorphological assessment of the osteoinductive capacity of a nanofibrillated cellulose-based composite and autologous blood clot

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Comparative histomorphological assessment of the osteoinductive capacity of a nanofibrillated cellulose-based composite and autologous blood clot

Purpose

The present study aimed to evaluate and compare the effect of nanofibrillated cellulose (NFC)-based composite with dicalcium phosphate dihydrate and an autologous blood clot (ABC) on the formation of new bone tissue in vivo by histological and histomorphometric assessment.

Materials and Methods

A total of 72 rats with created femoral defects (2 mm) were used. The rats were divided into three groups: (1) with filling of the defect with an ABC, (2) NFC-1—with filling of both the cortical plate and intramedullary space in the defect area, and (3) NFC-2—with filling of only the intramedullary space in the defect area. Histological and histomorphometric analysis was performed to assess the healing of the bone defect after 14, 30 and 60 days.

Results

Complete closure of the cortical plate defect was detected in the NFC-2 group on Day 30 (p < 0.0001). Moreover, in both NFC groups on the 30th and 60th days, ongoing osteogenesis was observed, characterized by a large volume of newly formed circular pattern bone tissue in the intramedullary space.

Сonclusion

This study demonstrated that the NFC-based composite, which is located below the level of the cortical plate, tamponing only the intramedullary space (NFC-2), improves bone tissue repair at the site of a bone defect of the cortical plate and has the potential of prolonged osteoinductivity.