通过激活抗氧化系统和相关基因的表达,使用不同药剂进行种子处理可减轻剑麻(Hibiscus cannabinus L.)的盐胁迫。

IF 3.4 3区 生物学 Q1 PLANT SCIENCES
Renxue Li, Dengjie Luo, Muzammal Rehman, Xin Li, Caijin Wang, Shan Cao, Guofeng Xu, Meng Wang, Canni Chen, Jingzhi Nie, Ru Li, Tao Chen, Peng Chen
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引用次数: 0

摘要

高盐度是一种限制作物产量的非生物胁迫。木槿(Hibiscus cannabinus L.)是锦葵科木槿属的一年生纤维作物,对盐胁迫有一定的耐受性。研究表明,种子引种可改善盐胁迫对植物的不利影响。然而,人们对经引诱剂处理过的剑麻种子的抗盐机理还不完全清楚。在本研究中,我们使用了四种不同浓度的引诱剂(H2O、PEG、ABA、KNO3)处理剑麻种子,并将诱导的剑麻幼苗置于盐胁迫(150 mM NaCl)条件下,测定其农艺性状和生理生化指标。结果表明,PEG 的最佳催芽浓度为 10%,ABA 为 0.5 μM,KNO3 为 0.5%。在这些处理浓度下,剑麻的发芽率显著提高,鲜重也分别增加了 35.1%、33.39%、20.78% 和 15.3%。此外,引种剂的使用还能在一定程度上缓解盐胁迫的不利影响,显著提高剑麻的株高、茎粗、叶面积等农艺指标,增强植株进行光合作用的能力,进一步提高抗氧化酶的活性和增加渗透物质的含量,减少细胞中 H2O2、O2 - 和 MDA 的积累。同时,引种还能提高参与盐胁迫途径的 HcSOS1、HcNHX、HcHKT、HcCBL、HcCIPK、HcPD 和 HcNCED 的表达。这些结果证明,种子引诱可以减少盐胁迫对剑麻的不利影响:在线版本包含补充材料,见 10.1007/s12298-024-01521-x。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Seed priming using different agents can alleviate salt stress in kenaf (Hibiscus cannabinus L.) by activating antioxidant system and related genes expression.

High salinity is an abiotic stress that limits crop production. Kenaf (Hibiscus cannabinus L.) is an annual fiber crop of the genus Hibiscus in the family Malvaceae with a certain tolerance to salt stress. Seed priming has been shown to ameliorate the adverse effects of salt stress on plants. However, the salt resistance mechanism in kenaf seeds treated with priming agents is not fully understood. In this study, we used four priming agents (H2O, PEG, ABA, KNO3) in different concentrations to treat kenaf seeds, and subjected the induced kenaf seedlings to salt stress (150 mM NaCl) to measure their agronomic traits and physiological and biochemical indicators. Our results indicate that the optimal priming concentration for PEG was 10%, 0.5 μM for ABA, and 0.5% for KNO3. Under these treatment concentrations, the germination rate of kenaf was significantly increased, and the fresh weight was also increased by 35.1%, 33.39%, 20.78% and 15.3%, respectively. Furthermore, the use of priming agents can alleviate the adverse effects of salt stress to a certain extent, significantly increase the agronomic indicators such as plant height, stem thickness, and leaf area of kenaf, enhance the ability of plants to perform photosynthesis, further improve the activity of antioxidant enzymes and increase the content of osmotic material, and reduce the accumulation of cell H2O2, O2 - and MDA. Meanwhile, seed priming can also enhance the expression of HcSOS1, HcNHX, HcHKT, HcCBL, HcCIPK, HcPD and HcNCED involved in the salt stress pathway. These results warrant that seed priming can reduce the adverse effects of salt stress on kenaf.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-024-01521-x.

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来源期刊
CiteScore
7.10
自引率
0.00%
发文量
126
期刊介绍: Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.
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