{"title":"超临界流体色谱法制备毫克级对映体。","authors":"Naomi Knavs, Alina Ghinet, Emmanuelle Lipka","doi":"10.1002/bmc.6030","DOIUrl":null,"url":null,"abstract":"<p><p>Preparative chromatographic enantioseparation is now the preferred technique for obtaining milligram quantities of pure enantiomers in the initial phase of development of a therapeutic compound. Supercritical fluid chromatography offers several advantages over liquid chromatography and was therefore selected for the preparative enantioseparation of a new potential anti-inflammatory molecule. Approximately 10 mg of each of the two enantiomers was successfully prepared using a Chiralpak AD-H (tris-3,5-dimethylphenylcarbamate of amylose) polysaccharide-based stationary phase with 40% of ethanol as a co-solvent, using a stacked injection mode. A peak distortion was observed during volume overloading, which may be due to the mixed-stream injection method.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":" ","pages":"e6030"},"PeriodicalIF":1.8000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Supercritical fluid chromatography for milligram preparation of enantiomers.\",\"authors\":\"Naomi Knavs, Alina Ghinet, Emmanuelle Lipka\",\"doi\":\"10.1002/bmc.6030\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Preparative chromatographic enantioseparation is now the preferred technique for obtaining milligram quantities of pure enantiomers in the initial phase of development of a therapeutic compound. Supercritical fluid chromatography offers several advantages over liquid chromatography and was therefore selected for the preparative enantioseparation of a new potential anti-inflammatory molecule. Approximately 10 mg of each of the two enantiomers was successfully prepared using a Chiralpak AD-H (tris-3,5-dimethylphenylcarbamate of amylose) polysaccharide-based stationary phase with 40% of ethanol as a co-solvent, using a stacked injection mode. A peak distortion was observed during volume overloading, which may be due to the mixed-stream injection method.</p>\",\"PeriodicalId\":8861,\"journal\":{\"name\":\"Biomedical Chromatography\",\"volume\":\" \",\"pages\":\"e6030\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-11-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Chromatography\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1002/bmc.6030\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/bmc.6030","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Supercritical fluid chromatography for milligram preparation of enantiomers.
Preparative chromatographic enantioseparation is now the preferred technique for obtaining milligram quantities of pure enantiomers in the initial phase of development of a therapeutic compound. Supercritical fluid chromatography offers several advantages over liquid chromatography and was therefore selected for the preparative enantioseparation of a new potential anti-inflammatory molecule. Approximately 10 mg of each of the two enantiomers was successfully prepared using a Chiralpak AD-H (tris-3,5-dimethylphenylcarbamate of amylose) polysaccharide-based stationary phase with 40% of ethanol as a co-solvent, using a stacked injection mode. A peak distortion was observed during volume overloading, which may be due to the mixed-stream injection method.
期刊介绍：
Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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