寨卡病毒 NS4B 蛋白 N 端区域在洗涤剂胶束中的 1H、15N 和 13C 骨架共振分配。

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2024-11-07 DOI:10.1007/s12104-024-10208-z

Yan Li, Ying Ru Loh, Qingxin Li, Dahai Luo, CongBao Kang

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引用次数: 0

摘要

寨卡病毒与小头畸形和成人格林-巴利综合征有关，已引起全球关注。病毒非结构蛋白之一--NS4B是一种整体膜蛋白，通过与病毒蛋白和宿主蛋白相互作用，在病毒复制过程中发挥着关键作用，使其成为抗病毒开发的一个有吸引力的药物靶点。我们纯化了 ZIKV NS4B 的 N 端区域（NS4B NTD），并将其重组到去垢胶束中。在此，我们报告了NS4B NTD在洗涤剂胶束中的骨架共振分配。现有的分配有助于了解其结构和配体结合，从而为开发 NS4B 抑制剂提供有用的信息。

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¹H, ¹⁵N and ¹³C backbone resonance assignment of the N-terminal region of Zika virus NS4B protein in detergent micelles.

Zika virus has raised global concerns due to its link to microcephaly and Guillain-Barré syndrome in adults. One of viral nonstructural proteins-NS4B, an integral membrane protein, plays crucial roles in viral replication by interacting with both viral and host proteins, rendering it an attractive drug target for antiviral development. We purified the N-terminal region of ZIKV NS4B (NS4B NTD) and reconstituted it into detergent micelles. Here, we report the assignments of the backbone resonances of NS4B NTD in detergent micelles. The available assignment is useful for understanding its structure and ligand binding to provide useful information for developing NS4B inhibitors.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.