Paola Capistrano dos Santos, Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Domickson Silva Costa, Marília Tedesco, Gabriela Tomas Jerônimo, Maurício Laterça Martins
{"title":"温度引起的用欧芹麻醉的尼罗罗非鱼血液学和生化指标的变化","authors":"Paola Capistrano dos Santos, Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Domickson Silva Costa, Marília Tedesco, Gabriela Tomas Jerônimo, Maurício Laterça Martins","doi":"10.1007/s10499-024-01751-6","DOIUrl":null,"url":null,"abstract":"<p>The study aimed to evaluate the effect of temperatures of 23 and 27 °C on the hematology of Nile tilapia juveniles (<i>Oreochromis niloticus</i>) subjected to anesthesia using the essential oil of basil (<i>Ocimum basilicum</i>) at a concentration of 250 mg L<sup>−1</sup>. A total of 112 fish were divided into 4 treatments and distributed as follows: fish anesthetized with 250 mg L<sup>−1</sup> at a temperature of 23 °C (<i>n</i> = 42); fish anesthetized with 250 mg L<sup>−1</sup> at a temperature of 27 °C (<i>n</i> = 42); and for each temperature, a control group fish exposed to diluent solution (95% ethanol) (<i>n</i> = 14, 7 per temperature); and a negative control group fish (water) (<i>n</i> = 14, 7 per temperature). They were exposed to 10 min for both temperatures. Blood collection was performed after the anesthetic induction at each temperature to determine the hematocrit, hemoglobin concentration (Hb), red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), relative values of leukocytes, and absolute values of leukocytes and thrombocytes, in addition to evaluating plasma chloride and total calcium ions. The analysis of chemical composition by gas chromatography coupled with mass spectrometry (GC–MS) demonstrated methyl-chavicol (70.04%) and linalool (24.59%) as the major components of basil essential oil. Fish anesthetized at a temperature of 23 °C showed lower neutrophil counts (<i>p</i> < 0.05) compared to the other tested groups. For the temperature of 27 °C, values of MCV, MCHC, and RBC decreased (<i>p</i> < 0.05) following exposure to basil essential oil. The calcium ion showed a statistical difference (<i>p</i> < 0.05) between the treatments about the water control group. It is concluded that basil essential oil presented a positive anesthetic effect. The use of <i>O. basilicum</i> essential oil as an anesthetic in Nile tilapia was the best at 23 °C, causing less hematological and biochemical imbalance.\n</p>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Temperature-induced changes in the hematological and biochemical parameters of Nile tilapia anesthetized with Ocimum basilicum\",\"authors\":\"Paola Capistrano dos Santos, Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Domickson Silva Costa, Marília Tedesco, Gabriela Tomas Jerônimo, Maurício Laterça Martins\",\"doi\":\"10.1007/s10499-024-01751-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The study aimed to evaluate the effect of temperatures of 23 and 27 °C on the hematology of Nile tilapia juveniles (<i>Oreochromis niloticus</i>) subjected to anesthesia using the essential oil of basil (<i>Ocimum basilicum</i>) at a concentration of 250 mg L<sup>−1</sup>. A total of 112 fish were divided into 4 treatments and distributed as follows: fish anesthetized with 250 mg L<sup>−1</sup> at a temperature of 23 °C (<i>n</i> = 42); fish anesthetized with 250 mg L<sup>−1</sup> at a temperature of 27 °C (<i>n</i> = 42); and for each temperature, a control group fish exposed to diluent solution (95% ethanol) (<i>n</i> = 14, 7 per temperature); and a negative control group fish (water) (<i>n</i> = 14, 7 per temperature). They were exposed to 10 min for both temperatures. Blood collection was performed after the anesthetic induction at each temperature to determine the hematocrit, hemoglobin concentration (Hb), red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), relative values of leukocytes, and absolute values of leukocytes and thrombocytes, in addition to evaluating plasma chloride and total calcium ions. The analysis of chemical composition by gas chromatography coupled with mass spectrometry (GC–MS) demonstrated methyl-chavicol (70.04%) and linalool (24.59%) as the major components of basil essential oil. Fish anesthetized at a temperature of 23 °C showed lower neutrophil counts (<i>p</i> < 0.05) compared to the other tested groups. For the temperature of 27 °C, values of MCV, MCHC, and RBC decreased (<i>p</i> < 0.05) following exposure to basil essential oil. The calcium ion showed a statistical difference (<i>p</i> < 0.05) between the treatments about the water control group. It is concluded that basil essential oil presented a positive anesthetic effect. The use of <i>O. basilicum</i> essential oil as an anesthetic in Nile tilapia was the best at 23 °C, causing less hematological and biochemical imbalance.\\n</p>\",\"PeriodicalId\":8122,\"journal\":{\"name\":\"Aquaculture International\",\"volume\":\"33 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-11-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture International\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10499-024-01751-6\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture International","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s10499-024-01751-6","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
Temperature-induced changes in the hematological and biochemical parameters of Nile tilapia anesthetized with Ocimum basilicum
The study aimed to evaluate the effect of temperatures of 23 and 27 °C on the hematology of Nile tilapia juveniles (Oreochromis niloticus) subjected to anesthesia using the essential oil of basil (Ocimum basilicum) at a concentration of 250 mg L−1. A total of 112 fish were divided into 4 treatments and distributed as follows: fish anesthetized with 250 mg L−1 at a temperature of 23 °C (n = 42); fish anesthetized with 250 mg L−1 at a temperature of 27 °C (n = 42); and for each temperature, a control group fish exposed to diluent solution (95% ethanol) (n = 14, 7 per temperature); and a negative control group fish (water) (n = 14, 7 per temperature). They were exposed to 10 min for both temperatures. Blood collection was performed after the anesthetic induction at each temperature to determine the hematocrit, hemoglobin concentration (Hb), red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), relative values of leukocytes, and absolute values of leukocytes and thrombocytes, in addition to evaluating plasma chloride and total calcium ions. The analysis of chemical composition by gas chromatography coupled with mass spectrometry (GC–MS) demonstrated methyl-chavicol (70.04%) and linalool (24.59%) as the major components of basil essential oil. Fish anesthetized at a temperature of 23 °C showed lower neutrophil counts (p < 0.05) compared to the other tested groups. For the temperature of 27 °C, values of MCV, MCHC, and RBC decreased (p < 0.05) following exposure to basil essential oil. The calcium ion showed a statistical difference (p < 0.05) between the treatments about the water control group. It is concluded that basil essential oil presented a positive anesthetic effect. The use of O. basilicum essential oil as an anesthetic in Nile tilapia was the best at 23 °C, causing less hematological and biochemical imbalance.
期刊介绍：
Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture.
The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more.
This is the official Journal of the European Aquaculture Society.
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