将硅藻代谢标定作为评估多瑙河两大支流水质的工具

IF 7 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecological Indicators Pub Date : 2024-11-01 DOI:10.1016/j.ecolind.2024.112793

Danijela Vidaković , Ntambwe Albert Serge Mayombo , Andrea Burfeid Castellanos , Michael Kloster , Bánk Beszteri

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引用次数: 0

摘要

本研究考察了数字显微镜和分子分析（rbcL 和 18S 标记）在评估两条国际河流（萨瓦河和蒂萨河）的硅藻群落组成和水质方面的有效性。此外，还采用了两种不同的方法来计算硅藻指数：基于类群的相对丰度和类群的存在/不存在。与 18S 相比，rbcL 标记更适合用来提供硅藻群落的全面信息，部分原因可能是参考数据库的覆盖范围存在差异。此外，形态分析和分子分析表明，所调查河流的生态状况在统计学上存在差异。根据分子数据得出的硅藻指数表明，与基于形态分析的硅藻指数相比，有一到两个级别的生态状况较差。不过，根据分子存在/不存在数据得出的硅藻平均指数要高于根据类群相对丰度得出的指数。在总结了所有结果之后，我们建议在大型低地河流的常规监测中使用 rbcL 标记和类群存在/不存在数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Diatom metabarcoding as a tool to assess the water quality of two large tributaries of the Danube River

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Diatom metabarcoding as a tool to assess the water quality of two large tributaries of the Danube River

This study examines the effectiveness of digital microscopic and molecular analysis (rbcL and 18S markers) in assessing diatom community composition and water quality in two international rivers (Sava and Tisa rivers). Also, two different approaches were applied for calculating diatom indices: based on the relative abundance of taxa and the presence/absence of taxa. The rbcL marker showed up as a more suitable choice for providing a comprehensive view of the diatom community compared to 18S, which can be partly attributed to variations in the coverage of reference databases. Furthermore, both morphological and molecular analysis pointed out statistically different ecological statuses of investigated rivers. Diatom indices derived from molecular data indicated one or two classes of lower ecological status than those based on morphological analysis. However, the average diatom indices derived from molecular presence/absence data were higher than those based on the relative abundance of taxa. After summarizing all the results, we can recommend using the rbcL marker with taxa presence/absence data for routine monitoring of large lowland rivers.

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来源期刊

Ecological Indicators 环境科学-环境科学

CiteScore

11.80

自引率

8.70%

发文量

1163

审稿时长

78 days

期刊介绍： The ultimate aim of Ecological Indicators is to integrate the monitoring and assessment of ecological and environmental indicators with management practices. The journal provides a forum for the discussion of the applied scientific development and review of traditional indicator approaches as well as for theoretical, modelling and quantitative applications such as index development. Research into the following areas will be published. • All aspects of ecological and environmental indicators and indices. • New indicators, and new approaches and methods for indicator development, testing and use. • Development and modelling of indices, e.g. application of indicator suites across multiple scales and resources. • Analysis and research of resource, system- and scale-specific indicators. • Methods for integration of social and other valuation metrics for the production of scientifically rigorous and politically-relevant assessments using indicator-based monitoring and assessment programs. • How research indicators can be transformed into direct application for management purposes. • Broader assessment objectives and methods, e.g. biodiversity, biological integrity, and sustainability, through the use of indicators. • Resource-specific indicators such as landscape, agroecosystems, forests, wetlands, etc.