研究寄生虫对果蝇长期影响的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-11-04 DOI:10.1016/j.xpro.2024.103438

Madhumala K Sadanandappa, Giovanni Bosco

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引用次数: 0

摘要

果蝇及其寄生虫为研究寄主-寄生虫生物学提供了一个生态相关模型，重点研究寄主防御策略和寄生虫对策中涉及的行为和生理反应。在此，我们概述了饲养蛹寄生蜂 Pachycrepoideus 的方案，以及评估寄生蜂暴露对成年果蝇长期影响的行为测定。我们详细介绍了准备果蝇并使其与黄蜂同居、记录产卵以及分析果蝇生殖反应和羽化的步骤。有关使用和执行该方案的完整细节，请参阅 Sadanandappa 等人的文章1。

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Protocol for studying parasitoid-induced long-term effects in Drosophila.

Drosophila and its parasitoids provide an ecologically relevant model for studying host-parasitoid biology, focusing on the behavioral and physiological responses involved in host defensive strategies and parasitoid countermeasures. Here, we outline a protocol for rearing Pachycrepoideus, a pupal parasitoid wasp, and a behavioral assay to assess the long-term impact of parasitoid exposure on adult Drosophila. We detail the steps for preparing and cohabiting Drosophila with the wasps, documenting egg-laying, and analyzing reproductive responses and eclosion in fruit flies. For complete details on the use and execution of this protocol, please refer to Sadanandappa et al.¹.

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