Highly Responsive Cryogel Based Sensing Platform by Encapsulating Programmed DNA for Colorimetric Detection of 17β-estradiol

Responsive hydrogels show obvious superiorities when being employed at detection owing to their polymeric networks and optical properties. Albeit important, the slow response of traditional hydrogels against targets limits their further applications, and enhancing the responsiveness of hydrogel is therefore imperative. Herein, a cryogel with hierarchical structures was designed through adjusting the hydrogel structure by the freezing process to improve the responsive properties, and fabricated for rapid analysis of 17β-estradiol (E2). The cryogel encapsulating the DNA hairpins rapidly adsorbed the complementary DNA isolated from the aptamer in the presence of E2 and activated an efficient hybridization chain reaction (HCR). After integration with a smartphone system, a portable colorimetric sensing platform was created for sensitive detection of E2 with a low detection limit of 0.5 nM. Our work provides a promising way for highly responsive hydrogel-based biosensor design, and broadens their applications in rapid and portable target detection.