比较添加不同水平的氯化锌、姜黄素、氧化锌纳米颗粒(Zano-NPs)和添加姜黄素的 Zano-NPs 对公羊精液解冻后质量的影响。

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES
Fatemeh Omidi, Hadi Hajarian, Hamed Karamishabankareh, Leila Soltani, Mojtaba Dashtizad
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引用次数: 0

摘要

研究目的本研究探讨了不同浓度的姜黄素(Curc)、氯化锌(ZnCl2)、氧化锌纳米颗粒(ZnO-NPs)和姜黄素负载于氧化锌纳米颗粒(Curc-co-ZnO-NPs)在冷冻稀释液中对解冻后公羊精子质量的影响:方法:使用小檗叶水提取物合成 ZnO-NPs。然后,在合成的 ZnO-NPs 上载入 Curc。我们使用分析方法来观察绿色合成 ZnO-NPs 和 Curc-co-ZnO-NPs 的成分、形态和大小,包括紫外可见光、ZETA 电位、EDX、DLS、FE-SEM 和 FT-IR。使用含有不同浓度 Curc、ZnCl2、ZnO-NPs 和 Curc-co-ZnO-NPs(0、1、10 和 100 µg/mL)的三碱扩展剂,将四种公羊的精液样本混合在一起。解冻后对各处理组的精子活力、存活率、DNA 和质膜完整性、总畸形率和丙二醛(MDA)生成情况进行了评估:结果表明,添加1 µg/mL的ZnO-NPs和Curc-co-ZnO-NPs能显著降低MDA和总畸变的水平(p 结论:ZnO-NPs和Curc-co-ZnO-NPs能显著降低MDA和总畸变的水平:冻融后,Curc-co-ZnO-NPs(1 µg/mL)似乎提高了精子参数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano-NPs), Curcumin Loaded on Zano-NPs on Post-Thawing Quality of Ram Semen.

Objective: This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl2), zinc oxide nanoparticles (ZnO-NPs) and Curc loaded on ZnO-NPs (Curc-co-ZnO-NPs) in cryopreservation dilution affected the quality of ram sperm after thawing.

Methods: ZnO-NPs were synthesised using Berberis vulgaris leaf aqueous extract. Then, Curc was loaded on the ZnO-NPs that had been synthesised. We used analytical methods to look at the composition, morphology and size of green synthesised ZnO-NPs and Curc-co-ZnO-NPs, including UV-Vis, zeta potential, EDX, DLS, FE-SEM and FT-IR. Using a Tris-base extender containing various concentrations of Curc, ZnCl2, ZnO-NPs and Curc-co-ZnO-NPs (0, 1, 10 and 100 µg/mL), semen samples from four rams were combined. Sperm motility, viability, DNA and plasma membrane integrity, total abnormalities and malondialdehyde (MDA) generation were all evaluated in treatment groups after thawing.

Results: The results showed that adding 1 µg/mL of ZnO-NPs and Curc-co-ZnO-NPs significantly reduced the level of MDA and total abnormalities (p < 0.05). Additionally, following the freeze-thawing procedure, the presence of 1 µg/mL of Curc-co-ZnO-NPs in the diluent of ram sperm significantly increased the percentage of sperm viability and motility in comparison to the control and other treatment groups (p < 0.05). Furthermore, as compared to the control group and other treatments, treatments containing 1 µg/mL of Curc-co-ZnO-NPs significantly improved membrane and DNA integrity (p < 0.05).

Conclusions: It appears that following freeze-thawing, the Curc-co-ZnO-NPs (1 µg/mL) enhanced sperm parameters.

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来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
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