miR-31b以芳基硫酸酯酶B为靶标,调控背带蝠的卵巢发育。

IF 3.8 1区 农林科学 Q1 AGRONOMY
Shan-Shan Yu, Qiang Zhang, Li-Yuan Zheng, Qian-Ping Xie, Jin-Jun Wang, Wei Dou
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引用次数: 0

摘要

背景:繁殖是昆虫种群增长和进化的基础,包括卵巢发育、繁殖行为和繁殖力。背腹扁虱是一种全球重要的农业害虫,需接受检疫,其交配雌虫可产卵 3000 多枚。卵巢发育的转录后调控仍不清楚。在这里,miR-31b 被证明参与调控背甲线虫的卵巢发育:结果:利用 CRISPR/Cas9 技术在蝙蝠卵巢发育过程中产生了 miR-31b 功能缺失突变。去除 miR-31b 会导致成虫卵巢发育严重受损,其表型包括产卵量和孵化率急剧下降。随后,利用生物信息学、转录组测序、定量聚合酶链式反应(qPCR)、RNA 下拉和双荧光素酶报告实验等方法,确定了 miR-31b 与其靶基因芳基硫酸酯酶 B(ARSB)之间的关系。最后,miR-31b 被证实与目标基因芳基硫酸酯酶 B 结合,影响新陈代谢,从而进一步阻碍了背甲双壳虫的卵巢发育:总之,这些结果为转录后水平调控卵巢发育和昆虫繁殖的分子机制提供了新的见解,从而为通过繁殖策略控制节肢动物害虫提供了潜在靶标。© 2024 化学工业协会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The miR-31b targets arylsulfatase B to regulate the ovarian development of Bactrocera dorsalis.

The miR-31b targets arylsulfatase B to regulate the ovarian development of Bactrocera dorsalis.

Background: Reproduction is the basis of insect population growth and evolution, and encompasses ovarian development, reproductive behavior, and fecundity. Bactrocera dorsalis is a globally significant agricultural pest that is subject to quarantine, with mated females that can lay over 3000 eggs. The post-transcriptional regulation of ovarian development remains unclear. Here, miR-31b is shown to be involved in regulating Bactrocera dorsalis ovarian development.

Results: CRISPR/Cas9 was used to generate miR-31b loss-of-function mutations in Bactrocera dorsalis. The removal of miR-31b resulted in severely impaired ovarian development in adults, with phenotypes that included dramatically reduced egg production and hatching rates. The relationship between miR-31b and its target gene arylsulfatase B (ARSB) was subsequently identified using the methods of bioinformatics, transcriptomic sequencing, quantitative polymerase chain reaction (qPCR), RNA pull-down and dual-luciferase reporter assay. Finally, miR-31b was confirmed to bind the target gene arylsulfatase B to affect metabolism and thereby further hindered ovarian development of Bactrocera dorsalis.

Conclusion: Overall, these results provide new insights into molecular mechanisms at the post-transcriptional level in regulating ovarian development and insect reproduction, consequently providing potential targets to control arthropod pests through the reproductive strategy. © 2024 Society of Chemical Industry.

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来源期刊
Pest Management Science
Pest Management Science 农林科学-昆虫学
CiteScore
7.90
自引率
9.80%
发文量
553
审稿时长
4.8 months
期刊介绍: Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.
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