一种基于发光的方案，用于检测线虫、小鼠和人类全血中的 NAD+/NADH。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-11-01 DOI:10.1016/j.xpro.2024.103428

He-Ling Wang, Jianying Zhang, Shu-Qin Cao, Maria Jose Lagartos-Donate, Shi-Qi Zhang, Sofie Lautrup, Zeping Hu, Costas A Lyssiotis, Riekelt H Houtkooper, Evandro F Fang

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引用次数: 0

摘要

在此，我们介绍一种 NAD+/NADH 检测方法，用于评估不同生物模型中的 NAD+、NADH 和 NAD+/NADH 比率，包括秀丽隐杆线虫、小鼠肌肉组织、小鼠全血和人类全血。我们介绍了不同模型的样本采集和制备步骤，以及 NAD+ 和 NADH 水平的检测和计算。该方案适用于不同生物模型的细胞/组织 NAD+ 和 NADH 水平的量化。

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A luminescent-based protocol for NAD⁺/NADH detection in C. elegans, mice, and human whole blood.

Here, we present a NAD⁺/NADH detection assay for evaluating NAD⁺, NADH, and NAD⁺/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD⁺ and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD⁺ and NADH levels across different biological models.

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