通过随机诱变研究一株泄殖肠杆菌对可乐定的异抗性机制。

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2024-12-01 DOI:10.1016/j.jgar.2024.10.260

Romane Milleville , Linda Hadjadj , Jean-Marc Rolain , Sophie Alexandra Baron

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引用次数: 0

摘要

泄殖腔肠杆菌复合菌由于经常出现异抗性，对可乐定的抗性没有很好的记录。之前的研究已经考察了双组分系统 PhoP-PhoQ 及其负调控因子 mgrB 或 arnBCADTEF 操作子的作用。我们利用基因组分析和随机诱变对一株泄殖腔肠杆菌进行了研究，确定了 arnA 基因对可乐定产生抗性的分子基础。

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Study of the mechanisms of colistin heteroresistance in a strain of Enterobacter cloacae by random mutagenesis

Objective

The Enterobacter cloacae complex belongs to a group in which colistin resistance is not well documented due to their frequent heteroresistance.

Methods

We investigated the molecular basis of colistin heteroresistance using genome analysis and random mutagenesis in a strain of E. cloacae.

Results

The arnA gene was truncated in the six colistin susceptible mutants we obtained. However, the mcr-9 gene was present in these mutants suggesting this gene is not responsible for colistin resistance.

Conclusions

Previous studies have highlighted the role of the two-component system PhoPQ and its negative regulator mgrB or the arnBCADTEF operon. Our results confirm the crucial role of this operon.

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.