{"title":"膳食中补充硫酸钠可改善蛋鸡的蛋壳质量、子宫离子运输和糖胺聚糖合成。","authors":"Kai-Bo Fu, Dong Dai, Jian-Min Zhou, Jing Wang, Hai-Jun Zhang, Shu-Geng Wu, Guang-Hai Qi, Jing Wang","doi":"10.5713/ab.24.0456","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the effects of dietary sodium sulfate (Na2SO4) supplementation on eggshell quality, uterine ion transportation, and glycosaminoglycan (GAG) synthesis.</p><p><strong>Methods: </strong>A total of 432 48-wk-old Hy-line Brown laying hens were randomly divided into 6 dietary treatments with 8 replicates of 9 birds each. The experimental laying hens were fed the corn-soybean meal diets (containing 0.15% NaCl) supplemented with 0.22%, 0.37%, 0.52%, 0.68%, 0.83%, or 0.99% Na2SO4 for 12 weeks.</p><p><strong>Results: </strong>Results showed that the eggshell breaking strength and eggshell ratio significantly increased in the 0.68% Na2SO4 group at the end of wk 56 and wk 60 (P < 0.05). In addition, eggshell thickness and weight significantly increased in the 0.68% Na2SO4 group at the end of wk 60 (P < 0.05). Eggshell calcium (Ca) content in the 0.68% Na2SO4 group was higher than that of 0.22% and 0.99% groups (P < 0.001). The concentrations of K+ and Ca2+ in the uterine fluid were significantly greater in the 0.68% group than in the other groups (P < 0.05). Dietary Na2SO4 increased the gene expression of SLC8A1, SCNN1A, ATP1B1, and KCNMA1 quadratically in the uterus (P < 0.05), and higher values were observed in 0.68% group. Additionally, the GAG contents of the eggshell, and ATP-S, SULT, CS, and DS contents of the isthmus increased linearly with the increment of dietary Na2SO4 (P < 0.05). There was a remarkable reduction in mammillary knob width, mammillary thickness, and the percentage of the mammillary layer (P < 0.05), and an increment in mammillary knob density, effective thickness, and total thickness in the 0.68% group compared with the 0.22% and 0.99% groups (P < 0.05).</p><p><strong>Conclusion: </strong>Overall, there was no dose-related difference with the increment of dietary Na2SO4 levels. The addition of 0.68% Na2SO4 in the corn-soybean basal diet (0.15% Cl) regulated uterine ion transport, increased GAG contents of eggshell, and improved eggshell ultrastructure and quality.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens.\",\"authors\":\"Kai-Bo Fu, Dong Dai, Jian-Min Zhou, Jing Wang, Hai-Jun Zhang, Shu-Geng Wu, Guang-Hai Qi, Jing Wang\",\"doi\":\"10.5713/ab.24.0456\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study evaluated the effects of dietary sodium sulfate (Na2SO4) supplementation on eggshell quality, uterine ion transportation, and glycosaminoglycan (GAG) synthesis.</p><p><strong>Methods: </strong>A total of 432 48-wk-old Hy-line Brown laying hens were randomly divided into 6 dietary treatments with 8 replicates of 9 birds each. The experimental laying hens were fed the corn-soybean meal diets (containing 0.15% NaCl) supplemented with 0.22%, 0.37%, 0.52%, 0.68%, 0.83%, or 0.99% Na2SO4 for 12 weeks.</p><p><strong>Results: </strong>Results showed that the eggshell breaking strength and eggshell ratio significantly increased in the 0.68% Na2SO4 group at the end of wk 56 and wk 60 (P < 0.05). In addition, eggshell thickness and weight significantly increased in the 0.68% Na2SO4 group at the end of wk 60 (P < 0.05). Eggshell calcium (Ca) content in the 0.68% Na2SO4 group was higher than that of 0.22% and 0.99% groups (P < 0.001). The concentrations of K+ and Ca2+ in the uterine fluid were significantly greater in the 0.68% group than in the other groups (P < 0.05). Dietary Na2SO4 increased the gene expression of SLC8A1, SCNN1A, ATP1B1, and KCNMA1 quadratically in the uterus (P < 0.05), and higher values were observed in 0.68% group. Additionally, the GAG contents of the eggshell, and ATP-S, SULT, CS, and DS contents of the isthmus increased linearly with the increment of dietary Na2SO4 (P < 0.05). There was a remarkable reduction in mammillary knob width, mammillary thickness, and the percentage of the mammillary layer (P < 0.05), and an increment in mammillary knob density, effective thickness, and total thickness in the 0.68% group compared with the 0.22% and 0.99% groups (P < 0.05).</p><p><strong>Conclusion: </strong>Overall, there was no dose-related difference with the increment of dietary Na2SO4 levels. The addition of 0.68% Na2SO4 in the corn-soybean basal diet (0.15% Cl) regulated uterine ion transport, increased GAG contents of eggshell, and improved eggshell ultrastructure and quality.</p>\",\"PeriodicalId\":7825,\"journal\":{\"name\":\"Animal Bioscience\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Bioscience\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.5713/ab.24.0456\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Bioscience","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5713/ab.24.0456","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens.
Objective: This study evaluated the effects of dietary sodium sulfate (Na2SO4) supplementation on eggshell quality, uterine ion transportation, and glycosaminoglycan (GAG) synthesis.
Methods: A total of 432 48-wk-old Hy-line Brown laying hens were randomly divided into 6 dietary treatments with 8 replicates of 9 birds each. The experimental laying hens were fed the corn-soybean meal diets (containing 0.15% NaCl) supplemented with 0.22%, 0.37%, 0.52%, 0.68%, 0.83%, or 0.99% Na2SO4 for 12 weeks.
Results: Results showed that the eggshell breaking strength and eggshell ratio significantly increased in the 0.68% Na2SO4 group at the end of wk 56 and wk 60 (P < 0.05). In addition, eggshell thickness and weight significantly increased in the 0.68% Na2SO4 group at the end of wk 60 (P < 0.05). Eggshell calcium (Ca) content in the 0.68% Na2SO4 group was higher than that of 0.22% and 0.99% groups (P < 0.001). The concentrations of K+ and Ca2+ in the uterine fluid were significantly greater in the 0.68% group than in the other groups (P < 0.05). Dietary Na2SO4 increased the gene expression of SLC8A1, SCNN1A, ATP1B1, and KCNMA1 quadratically in the uterus (P < 0.05), and higher values were observed in 0.68% group. Additionally, the GAG contents of the eggshell, and ATP-S, SULT, CS, and DS contents of the isthmus increased linearly with the increment of dietary Na2SO4 (P < 0.05). There was a remarkable reduction in mammillary knob width, mammillary thickness, and the percentage of the mammillary layer (P < 0.05), and an increment in mammillary knob density, effective thickness, and total thickness in the 0.68% group compared with the 0.22% and 0.99% groups (P < 0.05).
Conclusion: Overall, there was no dose-related difference with the increment of dietary Na2SO4 levels. The addition of 0.68% Na2SO4 in the corn-soybean basal diet (0.15% Cl) regulated uterine ion transport, increased GAG contents of eggshell, and improved eggshell ultrastructure and quality.
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